关键词:

肝移植 , 无肝期 , 动物模型 , 巴马小型猪

Abstract:

Objective To establish a liver transplantation anhepatic model in Bama miniature pigswith high repeatability and stability. Methods Five Bama miniature pigs were selected to construct a liver transplantation model in the anhepatic stage. In order to avoid intestinal congestion and circulatory failure in the anhepatic stage，a veno-venous bypass model was adopted to maintain stable blood pressure in the anhepatic stage， and to enhance intraoperative anesthesia and fluid management. The changes of hemodynamic indexes of intestinal injury，kidney injury and internal environment were observed and recorded at 6 time points ：30，60，90 min after VVB，before blood vessel occlusion and 30 min after vessel opening. Results The established veno-venous fluid transfer model of small pig liver transplantation in the anhepatic stage had adequate venous drainage. The

main results showed that 5 Bama small pigs were all alive，and the intestinal tube color was rosy and the peristalsis was normal，and the urine volume was normal. The operation time was（160±8）min，the blood loss was（158±32）ml，and the fluid rehydration was（1 760±251）ml. Compared with before vascular occlusion，the heart rate of the animals was significantly increased during vascular occlusion，the MAP was significantly decreased to（44±15）mmHg（1 mmHg ＝ 0.133 kPa），the blood pH was decreased，and the lactic acid was increased，heart rate slowed，and blood pH did not change significantly in lactic acid throughout the experiment，the experimentalanimals did not change significantly in electrolyte，while creatinine and intestinal fatty acid binding protein slowly increased. Conclusion The established Bama small porcine liver transplantation model is stable and safe，which can provide an effective animal model for further clinical trials such as validation of veno-venous transfer device. 

Key words:

Liver transplantation, Anhepatic stage, Animal model, Bama small pig