关键词:

肝移植 , 疾病器官模型 , 常温机械灌注 , 器官医学 , 肝脏劈离

Abstract:

Objective To develop a human liver splitting model with disease using normothermicmachine perfusion. Methods A liver from a liver transplant patient with acute-on-chronic liver failure and hepatitis B cirrhosis was split into left and right lobes，and the hepatic artery and portal vein of each half were respectively connected to two separate perfusion system for normothermic machine perfusion. Pressure and flow were continuously monitored during perfusion，and perfusate samples were collected for blood-gas analysis toassess the function of the diseased liver. Results Both lobes of the graft were successfully perfused in vitro for 16 h. Throughout this period，the pressure，flow，and other parameters of perfusion remained stable. The blood-gas analysis results of the perfusate were maintained within the physiological range. Conclusion We have successfully established a splitting perfusion model of diseased liver after liver transplantation that mimics human physiological and pathological conditions. This model allows for the detailed study of liver function under conditions that closelyapproximate human physiology. 

Key words:

"> Liver transplantation ； Diseased organ model ； Normothermic machine perfusion ； Organ medicine； Liver splitting