关键词:

肝移植 , 缺血 / 再灌注损伤 , 差异基因 , 靶点预测 , 炎症反应 , 核心基因 , 生物信息学分析

Abstract:

Objective To explore the molecular mechanisms and potential inflammation-related biomarkers of hepatic ischemia reperfusion injury（HIRI）process using bioinformatics techniques. Methods HIRI datasets GSE12720 and GSE14951 were obtained. Differential expressed genes（DEGs）were analyzed for functional enrichment and pathway enrichment. Hub genes were obtained using protein interactions analysis，and the correlationbetween Hub genes and inflammatory genes was calculated to finalize the key genes. The biological processes involved in these key genes were explored using GSEA analysis，and the expression of key genes was verified by mouse HIRI model. Finally，miRNAs of the key genes were predicted. Results Signaling pathways such as TNF，IL-17，MAPK and NF-κB were involved in inflammatory and immune processes during HIRI，and five key genes were closely related to inflammatory responses and their expressions are upregulated after HIRI. Conclusion This studyconfirms the central role of the inflammatory response in the process of HIRI and provides new perspectives for the discovery of the pathogenesis and potential therapeutic targets of HIRI. 

Key words:

Liver transplantation , Ischemia-reperfusion injury , Differential genes , Targetprediction, Inflammatory response, Core gene, Bioinformatics analysis