Objective The aim of our study is to develop a human hepatocellular carcinoma xenograft model in pigs and explore whether human cells could survive in pig liver，and to provide foundation information for clinical therapy of cancer in drug trials. Methods HepG2-Puro-GFP cells were injected into the Bama miniature pig’s liver which has been implemented partial hepatectomy by laparoscopic surgical procedures. Experiment were conducted in three groups（G0，G1 and G2）. Bama miniature pigs in G0 were injected with saline after implemented partial hepatectomy（sham）. Pigs in G1 were injected with human HepG2-Puro-GFP cells after implemented partial hepatectomy. Finally，pigs in G2 were also injected with human HeG2-Puro-GFP cells after implemented partial hepatectomy，and fed with immunosuppressant after surgery. The peripheral blood of each group was collected according to a certain time interval to detect the levels of alanine aminotransferase（ALT），aspartate transaminase （AST）and human alpha-feto protein（AFP）. Bama miniature pig’s liver tissues were harvested for hematoxylineosin（HE）staining to observe the morphologic features of the pig liver，and the survival of HepG2-Puro-GFcells were detected by immunohistochemistry staining. Results Levels of ALT and AST in each group restored to preoperative levels about 2 weeks after surgery. GFP-immunohistochemistry staining demonstrated that human HepG2 cells could exist in the G1 and G2 pig livers. Enzyme linked immunosorbent assay（ELISA）test indicated the expression of human AFP in the peripheral blood of the pigs in G1 and G2 groups. G2 group compared with G1 group, the difference was statistically significant〔AFP（μg/L）：39.54±20.30 vs. 13.77±7.52，P ＜ 0.05〕. HE staining showed the part of pigs had pathological changes in the liver，which included inflammatory cell infiltration and hepatocyte necrosis. Conclusion The pig model of human hepatocellular carcinoma can be achieved by orthotopic implantation of human hepatoma cells after partial hepatectomy. Immunosuppressant could prolong cells' survival time and survival efficiency.

Objective To explore the role of tumor necrosis factor-α-induced Protein-8-like2（TIPE2）in T cells mediated acute cardiac rejection in mouse. Methods Hematoxylin-eosin（HE）staining and flow cytometry were used to identify the preface of immune cells in cardiac allograft rejection. Immunofluorescence staining was taken to determine the relationship between TIPE2 and splenocytes. Real-time PCR was used to detect the expression of TIPE2 and cytokine in cardiac allograft. Results Seven days after heart transplantation of mice, the histological features of myocardia of allograft were extensive necrosis and abundant infiltration of inflammatory cells. Flow cytometry showed upregulations of CD4+ and CD8+ T cells in cardiac allograft. CD4+ or CD8+ staining in splenocytes also showed the expression of TIPE2. Real-time PCR detected increased expressions of TIPE2 and cytokine in cardiac allograft. Conclusion TIPE2 might take part in T cells mediated acute cardiac rejection process.

Objective To investigate the toxic effects of rapamycin and its derivatives, everolimus, cefotiam, zotarolimus on islets. Methods Experiments were performed on mouse insulinoma cells（MIN6）cells. MIN6 cells were cultured in a medium containing everolimus, diphospholimus, and zotarolimus for 48 hours, and cell proliferation was detected by Brdu. Cell viability was detected by CCK8, cell cycle was detected by PI, cell apoptosis was detected by flow cytometry, and insulin secretion by cells was detected by ELISA. The effects of three rapamycin derivatives on MIN6 cells were observed. Results We found that rapamycin derivatives impaired cell proliferation and viability. In the cell cycle and apoptosis experiments, the effect of three derivatives on MIN6 cells compared with the negative control, showed the trend of inhibited G1 phase to S phase transition and promoted apoptosis，but the difference had not statistically significant. So did Apotosis experiment. Morever，rapamycin derivatives treatment of MIN6 cells resulted in a loss of cell insulin secretion，the difference had statistically significant. Conclusion This report provides evidence that rapamycin derivatives ，as the same as the rapamycin ，had toxicity to islets.

Objective To analyze the relationship between the energy metabolism of donor liver before and post recanalization of blood vessels and the ischemic injury of bile duct injury after liver transplantation. Methods The data of patients who received liver transplantation in Tianjin First Center Hospital between March 2014 and August 2015 were analysed retrospectively. The patients who were satisfied with the standard were enrolled，and samples were taken before recanalization of portal vein，before recanalization of hepatic artery and 1 hour after recanalization of hepatic artery. Patients were followed for one year，patients enrolled were divided into ischemic-type biliary lesions（ITBL）group and non-ischemic-type biliary lesions（NITBL）group according to results of cholangiography. ATP，ADP and AMP at three timepoints in two groups were compared. Results There was no significance difference in energy metabolism showed by indices such as ATP，ADP and AMP， between ITBL group and NITBL group before recanalization of portal vein, before recanalization of hepatic artery and1 hour after recanalization of hepatic artery（P ＞ 0.05）. Conclusion There was no relationship between energy metabolism of donor liver and the occurrence of ITBL post liver transplantation.