Objective To explore the role of tumor necrosis factor-α-induced Protein-8-like2（TIPE2）in T cells mediated acute cardiac rejection in mouse. Methods Hematoxylin-eosin（HE）staining and flow cytometry were used to identify the preface of immune cells in cardiac allograft rejection. Immunofluorescence staining was taken to determine the relationship between TIPE2 and splenocytes. Real-time PCR was used to detect the expression of TIPE2 and cytokine in cardiac allograft. Results Seven days after heart transplantation of mice, the histological features of myocardia of allograft were extensive necrosis and abundant infiltration of inflammatory cells. Flow cytometry showed upregulations of CD4+ and CD8+ T cells in cardiac allograft. CD4+ or CD8+ staining in splenocytes also showed the expression of TIPE2. Real-time PCR detected increased expressions of TIPE2 and cytokine in cardiac allograft. Conclusion TIPE2 might take part in T cells mediated acute cardiac rejection process.

目的 探讨作为免疫负调控因子的肿瘤诱导坏死因子 -α- 诱导蛋白 8 样因子 -2（TIPE2） 在 T 细胞介导的小鼠心脏移植急性排斥反应中的潜在作用。方法 通过苏木素 - 伊红（HE）染色和流式细 胞术确定心脏移植物中的主要免疫排斥细胞，免疫荧光染色确定 TIPE2 与脾细胞间的关系，实时定量荧光 PCR（Real-time PCR）检测心脏移植物 TIPE2 及细胞因子表达量。结果 小鼠心脏移植 7 天后心脏组织出 现明显免疫细胞浸润及心肌组织的破坏，流式细胞术检测心脏移植物中 CD4 + 和 CD8+ T 细胞增高，免疫荧 光显示移植小鼠脾细胞中的 CD4+ 和 CD8+ T 细胞表达 TIPE2，而心脏移植物在第 7 天时 TIPE2 和细胞因子 表达量均明显增高。结论 TIPE2 与在 T 细胞介导的小鼠心脏移植急性排斥反应中可能发挥着潜在作用。