Objective Gene detection and immune microenvironment detection in liver cancer can guideanti-tumor therapy，but there are few studies on gene detection and microenvironment detection in liver cancer. In this study，we reported the results of two cases of hepatic carcinoma，one is hepatocellular carcinoma and the other is intrahepatic cholangiocarcinoma，and provided preliminary insightsfor the treatment of hepatic carcinoma in the future. Methods In this study，thetumorspecimensoftwopatientsweretestedby multipleximmunohistochemistry/immunofluorescence techniques and tumor gene sequencing. Both patients were under follow- up observation. Results Both patients were belonged to immune non-response type. The overall results showed that the effect of single drug immunotherapy may not be ideal. Partial gene detection results suggested that immune checkpoint blockade may be effective for both patients . Neither patient received immunotherapy. Conclusion The results of these two patients suggested that the effect of immunotherapy is not satisfactory. But immunotherapy is a promisingtreatment for cancerat present, and combinedimmunotherapy maybe helpful for liver cancer. 

Objective To explore the effects of different biopsy location and methods on the histopathological evaluation of donation after citizen's death（DCD）donor kidney. Methods Ten cases of discarded donor kidneys were collected from 2019 to 2022. Samples were harvested from different sites and preparedby paraffin section. After being stained with hematoxylin-eosin（HE），the number of glomeruli and arterioles and the degree of lesion were compared. The specimens were evaluated according to Banff score，Remuzzi score， Maryland score and Pirani score. The samples were compared with the other samples collected bycore needle biopsy，and the number of glomeruli and arterioles per unit area of the two samples were calculated. Results There was no difference in the number and degree of glomeruli and arterioles observed in specimens taken from differentparts（P ＞ 0.05），and there was no significant difference in Banff score，Remuzzi score，Maryland score and Pirani score（P ＞ 0.05）. The proportion of glomerulosclerosis（42%±8.8% vs. 25%±23.2%），intimal thickening of arterioles（68%±27% vs. 46.5%±22.8%）and arterioles hyaline degeneration（86%±17.4% vs. 59.3%±16.4%）incore needle biopsy specimens was higher than that in anatomical specimens. In the aspect of donor kidney scoring，only the degree of glomerulosclerosis in Banff score was significantly different between the two samples（2.2 ± 0.4 vs. 1.6 ± 0.9）， and there was no significant difference in other scoring systems（P ＞ 0.05）. The number of glomeruli in core needle

biopsy specimens per unit area was less than that in anatomical specimens〔（199.3±50.7）/cm2 vs.（240.6±57.4）/cm2〕，but the number of arterioles was greater than that in anatomical specimens〔（153.5±76.9）/cm2 vs.（114.9±43.7）/cm2〕. Conclusion The way of obtaining biopsy specimens from different parts had no effect on the histological evaluationof DCD donor kidney. The scoring results of core needle biopsy specimens can reflect the degree of donor kidney lesions. The sample getting from core needle biopsy is deep，and the degree of angle entering the donor kidney should be appropriately decreased.