Objective Post-renal transplantation anemia（PTA）occurs frequently in kidney transplant recipients，significantly impacting their quality of life and graft loss. Currently，effective methods to predictthe risk of persistent PTA early post-transplantation are lacking. This study aimed to develop a nomogram prediction model for early persistent PTA specifically tailored to kidney transplant recipients. Methods Using the electronic medical record system of Southern Hospital of Southern Medical University，patient data from January 1，2020 to December 31，2022 were obtained，and 245 subjects were ultimately selected as the research subjects. Among these，85% were randomly selected as the training set for model development，and the remaining 15% constituted the testing set. Using the Least Absolute Shrinkage and Selection Operator（Lasso）regression model，variables potentially affecting early persistent PTA were screened to identify predictive factors.A logistic regression analysis was employed to establish the prediction model. Model performance was assessed using Receiver operating characteristic（ROC）curves，area under the curve（AUC），Calibration plots，and decision Curve Analysis（DCA）. Results Identified predictive factors after screening included recipient's preoperative body mass index，preoperative serum albumin level，preoperative hemoglobin level，preoperative mean corpuscular volume，perioperative use of angiotensin-converting enzyme inhibitors or angiotensin receptor blockers，exogenous iron supplementation，and exogenous erythropoietin supplementation. The model demonstrated good discriminativeability with an AUC of 0.87 for the training set and 0.75 for the testing set，indicating robust predictive performance. Calibration and DCA further confirmed the accuracy and clinical utility of the model. Conclusion This nomogram prediction model utilizes early recipient information，including demographic characteristics，laboratory data，and medication regimens，to accurately predict individualized risk of early persistent PTA in kidney transplant recipients. This provides a basis for early clinical intervention，potentially improving patient prognosis and quality of life. 

Objective To explore the clinical effects of retroperitoneal laparoscopic donor nephrectomy and open donor nephrectomy. Methods The clinical data of donors from January 2023 to April 2024 were analyzed retrospectively， including 46 males and 93 females， with an average age of （54.21±6.52） years. Retroperitoneal laparoscopic nephrectomy was used in 99 cases and open nephrectomy was used in 40 cases. Data of donor's past history，kidney position， donor's renal artery length， kidney dimension， number of arteries， operation time， intraoperative bloodloss， surgical incision length， postoperative maximum creatinine， postoperative maximum urea nitrogen， postoperative hospitalization days， postoperative total drainage， donor's renal artery length after removal， warm ischemia time， intraoperative peritoneal damage and fat adhesion， and complications were collected and analyzed. Results Comparedwith the open operation group， the incision of laparoscopic operation was significantly reduced， the amount of bleeding and postoperative drainage were significantly reduced， the hospital stay of donors was significantly shortened （P ＜ 0.05），and the warm ischemia time was significantly prolonged （P ＜ 0.05）. Male， larger body mass index， previous smoking history， trauma history， larger kidney dimension， excessive bleeding during operation， peritoneal damage and fat adhesion significantly increased the operation time （P ＜ 0.05）. Conclusion On the premise of ensuring the quality of the kidney， laparoscopic operation has the advantages of small incision， less bleeding and quick recovery. 

Objective To investigate the role of fission protein 1 （FIS1） in affecting renal ischemiareperfusion injury by regulating mitochondrial division and apoptosis. Methods Probing FIS1 expression levels and apoptosis levels were measured at different times in the renal tubular epithelial cell（ HK-2）with hypoxiareoxygenation（ HR）model and mouse renal ischemia-reperfusion（ IR） model. Cell lines with FIS1 knockdown and overexpression were constructed，changes in the degree of mitochondrial division were observed using mitochondrial probes，and changes in the level of apoptosis were detected with flow cytometry. Results FIS1 knockdown/ overexpression had essentially no effect in normal cells. After HR， knockdown of FIS1 inhibited mitochondrial division and reduced apoptosis levels， and vice versa after overexpression of FIS1. Conclusion In IRI， Inhibition of FIS1 expression reduces mitochondrial division and reduces the level of apoptosis， which is expected to be a potential therapeutic target for IRI. 

Objective To investigate the protective effect of berberine pretreatment on liver injury in rats undergoing autogenous orthotopic liver transplantation and its related mechanism. Methods Twenty-four cleangrade healthy male Sprague-Dawley rats were divided into 3 groups（n ＝ 8）. They were sham operation group（S group），model group（AT group）and berberine pretreatment group（B group）. The weight was 250 ～ 280 g. In S group，the abdomen was opened，the corresponding blood vessels and ligaments were isolated，and the abdomen wasclosed. Rat models of liver injury after orthotopic liver transplantation were prepared in both AT and B groups，andberberine〔200 mg/（kg·d）〕was given by gavage 1 week before surgery in B group. Rats were anesthetized at 6 h after reperfusion，and the serum and liver tissues were collected. The serum levels of alanine aminotransferase（ALT）and aspartate aminotransferase（AST）were detected，the contents of serum high mobility group box-1 protein（HMGB1）（by enzyme-linked immunosorbent assay），and the expression of peroxisome proliferator activated receptor（PPARγ） and nuclear transcription factor-κB（NF-κB）（by immunohistochemistry）were determined. The expressions of ASC，NLRP3 and Pro-caspase1（by Western blot）were detected. Results Compared with S group，the levelsof serum ALT and AST and the contents of HMGB1 were significantly increased. The expression of PPARγin liver was down-regulated and the expression NF-κB、ASC、NLRP3 and Pro-caspase1 in liver was up-regulated，and histopathological injury of liver was significantly aggravated in AT and B groups（P ＜ 0.05）. Compared with AT group，the levels of serum ALT and AST and the contents of HMGB1 were significantly decreased. The expression of PPARγin liver was up-regulated and the expression NF-κB、ASC、NLRP3 and Pro-caspase1 in liver wasdown-regulated in liver tissue（P ＜ 0.05），and liver histopathological damage was significantly improved in B group. Conclusion Pretreatment of berberine can inhibit the expression of NF-κB by activating PPARγand then inhibiting the pyroptosis pathway，thus play a protective role against liver injury induced by liver transplantation in rats. 

Objective Establish model of abdominal heterotopic heart transplantation in mice and summarize the experience to provide animal model support for further study of organ transplantation immunology. Methods Inbred BALB/c（n ＝ 30）and C57BL/6（n ＝ 30）mice were selected as donors，and inbred BALB/c（n ＝ 60）mice were used as recipients. The ascending aorta of the donor was anastomosed to the abdominal aorta of the recipient，and the pulmonary artery of the donor was anastomosed to the inferior vena cava of the recipient respectively to establish the heterotopic heart transplantation model. The survival time and the rejection of grafts were observed postoperatively. Results The successful rate of transplantation was 85%（51/60）. The donoroperation time was（7.0±1.0）min，and the recipient operation time was（60±10）min. The vascular anastomosis time was（25±3.0）min. After the transplantation，no immunosuppressive agent was used，and the survival time of the graft was（7.6±0.9）d. The graft on the fifth day，the seventh day showed typical rejection by histopathology. Conclusion Skilled microsurgical techniques and timely management of surgical complications are key to the successful establishment of abdominal heterotopic heart transplantation in mice. 

Objective To observe the efficacy and survival status of single donor kidney transplantation with high Remuzzi score at zero-point biopsy. Methods A retrospective analysis was conducted on 178 recipients of single donor kidney transplantation who received deceased organ donation at the Second People's Hospital of Shanxi Province from January 2018 to January 2021. The donor kidneys underwent zeropoint biopsies and were evaluated with pathological Remuzzi scoring. The recipients were divided into high scoring group（≥ 4 and ≤ 6）and low scoring group（≤ 3）. The occurrence of delayed graft function of transplanted kidneys，postoperative renal function，occurrence of proteinuria，and survival of recipients and transplantedkidneys in both groups were observed with a follow-up time of 36 months. Results There were no statisticallysignificant differences（P ＞ 0.05）in gender ratio，body mass index，human leukocyte antigen（HLA）mismatch number，and donor kidney cold ischemia time between the two groups of recipients; there was no statistically significant difference in baseline blood creatinine and glomerular filtration rate before surgery（P ＞ 0.05）. A total number of 21 cases（23.6%）in the high scoring group experienced delayed graft function of transplanted kidneys after surgery，while 6 cases（6.7%）in the low scoring group experienced delayed graft function. The difference between the two groups was statistically significant（P ＜ 0.05），24 cases（27%）in the high scoring group developed proteinuria after surgery，while 9 cases（10.1%）developed proteinuria in the low scoring group. Through multiple factor analysis，it was found that the occurrence of proteinuria after kidney transplantation and the addition of mTOR immunosuppressants after surgery （OR ＝ 4.52， P ＜ 0.05）were related to thepreoperative Remuzzi score（OR ＝ 1.46，P ＜ 0.05）. At a follow-up of 36 months，the high scoring group had a blood creatinine level of（131.3±5.53）μmol/L and an eGFR level of（62.9±2.02）ml/（min · 1.73 m2 ）， while the low scoring group had a blood creatinine level of（121.3±2.18）μmol/L and an eGFR level of（65.0± 1.24）ml/（min·1.73 m2 ）. There was no statistically significant difference between the two groups（P ＞ 0.05）. Thesurvival rate of recipients in the high scoring group 36 months after surgery was 95.5%（85 cases），and the survival rate of transplanted kidneys was 95.5%（85 cases）. The survival rate of recipients in the low scoring group was 95.5% （85 cases），and the survival rate of transplanted kidneys was 97.7%（87 cases），with no statistically significant difference（P ＞ 0.05）. Conclusion Single kidney transplantation with a pre-transplant renal biopsy score of 6 ≥ Remuzzi ≥ 4 can achieve good long-term kidney survival and is worthy of clinical implementation.