Objective To investigate the role of fission protein 1 （FIS1） in affecting renal ischemiareperfusion injury by regulating mitochondrial division and apoptosis. Methods Probing FIS1 expression levels and apoptosis levels were measured at different times in the renal tubular epithelial cell（ HK-2）with hypoxiareoxygenation（ HR）model and mouse renal ischemia-reperfusion（ IR） model. Cell lines with FIS1 knockdown and overexpression were constructed，changes in the degree of mitochondrial division were observed using mitochondrial probes，and changes in the level of apoptosis were detected with flow cytometry. Results FIS1 knockdown/ overexpression had essentially no effect in normal cells. After HR， knockdown of FIS1 inhibited mitochondrial division and reduced apoptosis levels， and vice versa after overexpression of FIS1. Conclusion In IRI， Inhibition of FIS1 expression reduces mitochondrial division and reduces the level of apoptosis， which is expected to be a potential therapeutic target for IRI. 

Objective To explore a common cause of delayed graft function（DGF）and its diagnosis and treatment methods. Methods One patient after renal transplantation were reported，who experience hump shape change of urine up to 2 months，and the related literature of the disease were reviewed toanalyze the causes of DGF. Results The allograft kidney transplantation patient show a hump shape change of urine，and was treated by porcine immunoglobulin of anti-human T cells，and Danshen andChuanqongqin injection improving micro circulation. Regular hemodialysis was performed to remove the body of inflammatory mediators，and water， electrolyte and acid-base balance were maintained，and infection prevention and support treatment were done. Patient was discharged as urine output was gradually stabilized about 2 000 mL，and serum creatine around 130 μmol/LConclusion Full awareness of the common causes of DGF is helpful to reduce the incidence of DGF. In case of occurrence，early blood purification and auxiliary measures should be performed，and most DGF can recover.

Objective To investigate the significance of color doppler ultrasound for detecting hepatic arteryin pediatric liver transplantation. Methods Application of color doppler ultrasound to observe the hepatic artery in57 pediatric after liver transplantation，observe whether the signal of blood flow，systolicvelocity（S），diastolic velocity （D）and S/D. The minimum follow-up time is 2 months，the longest is 9 months. All patients with artery detection data were collected for analysis. Results No obvious abnormality of hepatic artery was observed in 52 cases with colordoppler ultrasound. Arterial flow signal could not be detected in 5 patients 5-8 days after transplantation and hepaticartery thrombosis was considered，in which 3 cases were confirmed by digital subtraction angiography（DSA），1 case by contrast-enhanced ultrasound and CT，and 1 case was confirmed to be ultrasonic false positive with DSA showedno obvious abnormal hepatic artery. Diagnosis coincidence rate of color doppler ultrasound in hepatic artery detection was 98%. Conclusion Color Doppler ultrasound provides an important basis for hepatic artery monitoring and early diagnosis of complication after liver transplantation.

Objective To evaluate the efficacy and safety of bicyclol treatment in drug-induced liver injuryafter renal transplantation. Methods A retrospective analysis was performed on 36 cases of renal transplantation， who experienced drug-induced liver injury 3 months after transplantation from January 2010 to August 2013 in Second People's Hospital of Shanxi Province. 22 cases from October 2011 to August 2013 were served as test group （treated with bicyclol 25 mg/50 mg）, while 14 cases from January 2010 to September 2011 as control group（treated with capsule of Gan Caosuaneran 150 mg）. The patients in two groups received treatment 3 times per day for 4 weeks.Before and 4 weeks after treatment, the changes in alanine aminotransferase（ALT）, aspartateaminotransferase （AST）, total bilirubin（TBil）and serum creatinine（SCr）were recorded，as well as 50% decreased time and restore tonormal time of ALT. Adverse reaction induced by drugs was observed. Results ALT，AST and TBil after treatment in both groups were significantly lower than those before treatment（all P ＜ 0.05），but there was no significant change in SCr. There were no significant differences in above indexes before and after treatment between two groups （all P ＞ 0.05）. The average time of 50％ decrease in ALT and the time of return to normal in test group were significantly shorter than those in control group〔time of decreased 50％（days）：5.30±2.26 vs. 11.00±5.36，timeof return to normal（days）：7.60±4.71 vs. 16.00±9.71，both P ＜ 0.05〕. During treatment，there was no rejectionor drug-related adverse reaction in both groups. Conclusion The efficacy of bicyclol treatment on drug-induced liver injury after renal transplantation is precise，safe，and easy to use。

Objective To investigate the methods and techniques for organ procurement from Chinesefirst category infant donation after cardiac death，and to evaluate post-transplant outcomes. Methods In this retrospective study, clinical data of 6 cases of Chinese first category infant donation after cardiac death organprocurements were analyzed Modified epigastric multivisceral organ harvesting were performed in all of thecases. Results The average warm ischemia time was 2 minutes（1.0 ～ 3.6 minutes）with the average procurementtime of 46 minutes（36 ～ 58 minutes）. Six livers and 12 kidneys were procured followed by 6 cases of livertransplantation，6 cases of en-bloc kidney transplantation. All of the recipients were healthy for 3 ~ 16 months follow-up without primary non-function and other complications except one who underwent transplant nephrectomy due to renal vein thrombosis on post operation day 7. Conclusion Modified epigastric multivisceral organ harvesting is a safe method for organ procurement from Chinese first category infant donation after cardiac deathguaranteeing procurement of high quality of organs.