Objective To investigate the role of fission protein 1 （FIS1） in affecting renal ischemiareperfusion injury by regulating mitochondrial division and apoptosis. Methods Probing FIS1 expression levels and apoptosis levels were measured at different times in the renal tubular epithelial cell（ HK-2）with hypoxiareoxygenation（ HR）model and mouse renal ischemia-reperfusion（ IR） model. Cell lines with FIS1 knockdown and overexpression were constructed，changes in the degree of mitochondrial division were observed using mitochondrial probes，and changes in the level of apoptosis were detected with flow cytometry. Results FIS1 knockdown/ overexpression had essentially no effect in normal cells. After HR， knockdown of FIS1 inhibited mitochondrial division and reduced apoptosis levels， and vice versa after overexpression of FIS1. Conclusion In IRI， Inhibition of FIS1 expression reduces mitochondrial division and reduces the level of apoptosis， which is expected to be a potential therapeutic target for IRI. 

Objective To summarize the clinical characteristics，diagnosis，treatment and prognosis ofdigestive system malignancy following renal transplantation. Methods The clinical data on fifty-six cases of digestive system malignancy followingrenal transplantationbetween Jan 2000 and July2022 was retrospectively analyzed．Results Among the fifty-sixcases，forty-fourweremalesandtwelve were females.Twoof them had esophageal cancer，ten had gastric carcinoma，twenty-six had liver cancer，three had pancreatic carcinoma，two had tumor of duodenalampulla，and thirteen had intestinal cancer. The diagnosis was made based on the clinical symptoms，laboratory andimaging findings of the patients during follow-up. The patients receivedcomprehensive treatment mainly with surgery. The survival time of patients ranged from one month to eleven years. Conclusion There wasacloserelationshipbetween digestive system tumor and virus infection in renal transplantrecipients. Regular tumor screening after transplantationwas helpful for early diagnosis. According to the characteristics of the tumor，early and appropriate treatment measures should betaken.

Objective To investigate the predictive value of admission red blood cell distribution width （RDW）for early postoperative major adverse events in heart transplant recipients. Methods Clinical data of 57 patients who underwent heart transplantation at Central China Fuwai Hospital of Zhengzhou University between April 2018 and December 2024 were retrospectively analyzed. Patients were stratified into a favorable prognosis group （n ＝ 44）and an adverse prognosis group（n ＝ 13）based on the occurrence of major adverse events within 30 days postoperatively（all-cause death，reoperation for any cause，requirement for extracorporeal membrane oxygenation，or new-onset continuous renal replacement therapy）. Risk factors were screened using logistic regression analysis，and predictive performance was evaluated using receiver operating characteristic（ROC）curves. Results AdmissionRDW was significantly higher in the adverse prognosis group compared to the favorable prognosis group（15.69 ±1.32% vs. 14.70±1.56%，P ＝ 0.043）. Multivariate logistic regression analysis identified admission RDW as an independent risk factor for early postoperative adverse events（OR ＝ 1.875，95% CI ＝ 1.053 ～ 3.341，P ＝ 0.033）. The area under the ROC curve was 0.704（P ＝ 0.027），with an optimal cutoff value of 15.5%（sensitivity was 61.5%，specificity was 75.0%）. Conclusion Elevated admission RDW independently correlates with early major adverse events following heart transplantation and may serve as a potential biomarker for preoperative risk assessment. 

obtained by clippingSD rat thoracic aorta for 30 minutes as warm ischemia；rat NMP system was established in vitro. According to differentmethods of preservation of the donor livers，the experiment was divided into: Normal group（n ＝ 6），serum and livers were retained for use ；NMP group（n ＝ 30），livers were collected after 4，6，and 8 h，outflow and inflow perfusatewas collected at 0，2，4，6 and 8 h after infusion for testing ；static cold storage（SCS）group（n ＝ 6），the livers were flushed out of the blood with 20 ml 4 ℃ UW solution and were collected after 6 h of SCS in UW solution at 4 ℃ .Liver function in outflow perfusate was detected by biochemical methods ；liver tissue histopathology was observed by hematoxylin-eosin staining ；hepatocyte ultrastructure was observed by transmission electron microscopy ；hepatocyteapoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling ；endothelin-1，endothelialnitric oxide synthase（eNOS），inducible nitric oxide synthase（iNOS），von Willebrand factor（vWF），intercellular adhesion molecule（ICAM-1）and intervascular adhesion molecule -1 （VCAM-1）expression were detected byWestern blot. Results Compared with SCS，NMP significantly improved the histological damage of DCD donorlivers，the Suzuki’s score of NMP group（3.40±0.55）is significantly lower than that of SCS group（7.00±0.71，F ＝ 229.75，P ＜ 0.05）；reduced hepatocytes apoptosis，the number of apoptotic cells in the NMP group（9.80±1.48）was significantly lower than that in the SCS group（33.40±4.39，F ＝ 166.58，P ＜ 0.05）；meanwhile，NMP could repair liver mitochondrial damage，the number of irreversible damaged mitochondria in the NMP group（1.60±0.55）was significantly lower in the SCS group（2.80±0.45，F ＝ 36.29，P ＜ 0.05）. It was further found that NMPcould improve DCD liver through: ① Inhibiting intercellular adhesion and improving endothelial cell damage ；compared with SCS，NMP significantly inhibited the expression of ICAM-1（F ＝ 1728.45，P ＜ 0.05），VCAM-1 （F ＝ 254.72，P ＜ 0.05）and vWF（F ＝ 595.30，P ＜ 0.05）in the liver. ② Improving liver ET-1 / NOS balance and microcirculation perfusion ；compared with SCS，NMP significantly inhibited the expression of ET-1（F ＝ 1372.51，P ＜ 0.05）and iNOS（F ＝ 1102.20，P ＜ 0.05）in the liver and promoted the expression of eNOS（F ＝ 271.66，P ＜ 0.05）. Conclusion NMP could improve the microcirculation of DCD donor liver and improve the quality of donor liver. The mechanism may rely on its role in inhibiting intercellular adhesion，improving sinusoidal endothelialinjury and microcirculation perfusion. 

Objective To evaluate the efficacy and safety of bicyclol treatment in drug-induced liver injuryafter renal transplantation. Methods A retrospective analysis was performed on 36 cases of renal transplantation， who experienced drug-induced liver injury 3 months after transplantation from January 2010 to August 2013 in Second People's Hospital of Shanxi Province. 22 cases from October 2011 to August 2013 were served as test group （treated with bicyclol 25 mg/50 mg）, while 14 cases from January 2010 to September 2011 as control group（treated with capsule of Gan Caosuaneran 150 mg）. The patients in two groups received treatment 3 times per day for 4 weeks.Before and 4 weeks after treatment, the changes in alanine aminotransferase（ALT）, aspartateaminotransferase （AST）, total bilirubin（TBil）and serum creatinine（SCr）were recorded，as well as 50% decreased time and restore tonormal time of ALT. Adverse reaction induced by drugs was observed. Results ALT，AST and TBil after treatment in both groups were significantly lower than those before treatment（all P ＜ 0.05），but there was no significant change in SCr. There were no significant differences in above indexes before and after treatment between two groups （all P ＞ 0.05）. The average time of 50％ decrease in ALT and the time of return to normal in test group were significantly shorter than those in control group〔time of decreased 50％（days）：5.30±2.26 vs. 11.00±5.36，timeof return to normal（days）：7.60±4.71 vs. 16.00±9.71，both P ＜ 0.05〕. During treatment，there was no rejectionor drug-related adverse reaction in both groups. Conclusion The efficacy of bicyclol treatment on drug-induced liver injury after renal transplantation is precise，safe，and easy to use。

Objective To investigate the methods and techniques for organ procurement from Chinesefirst category infant donation after cardiac death，and to evaluate post-transplant outcomes. Methods In this retrospective study, clinical data of 6 cases of Chinese first category infant donation after cardiac death organprocurements were analyzed Modified epigastric multivisceral organ harvesting were performed in all of thecases. Results The average warm ischemia time was 2 minutes（1.0 ～ 3.6 minutes）with the average procurementtime of 46 minutes（36 ～ 58 minutes）. Six livers and 12 kidneys were procured followed by 6 cases of livertransplantation，6 cases of en-bloc kidney transplantation. All of the recipients were healthy for 3 ~ 16 months follow-up without primary non-function and other complications except one who underwent transplant nephrectomy due to renal vein thrombosis on post operation day 7. Conclusion Modified epigastric multivisceral organ harvesting is a safe method for organ procurement from Chinese first category infant donation after cardiac deathguaranteeing procurement of high quality of organs.