Abstract:

Objective Gene detection and immune microenvironment detection in liver cancer can guideanti-tumor therapy，but there are few studies on gene detection and microenvironment detection in liver cancer. In this study，we reported the results of two cases of hepatic carcinoma，one is hepatocellular carcinoma and the other is intrahepatic cholangiocarcinoma，and provided preliminary insightsfor the treatment of hepatic carcinoma in the future. Methods In this study，thetumorspecimensoftwopatientsweretestedby multipleximmunohistochemistry/immunofluorescence techniques and tumor gene sequencing. Both patients were under follow- up observation. Results Both patients were belonged to immune non-response type. The overall results showed that the effect of single drug immunotherapy may not be ideal. Partial gene detection results suggested that immune checkpoint blockade may be effective for both patients . Neither patient received immunotherapy. Conclusion The results of these two patients suggested that the effect of immunotherapy is not satisfactory. But immunotherapy is a promisingtreatment for cancerat present, and combinedimmunotherapy maybe helpful for liver cancer. 

Key words:

Tumor microenvironment ；Immune checkpoints ；Immunotherapy ；Genedetection ；Combined immunotherapy for cancer ,

摘要：

目的 肝癌的基因突变筛查和免疫微环境检测可指导抗肿瘤治疗，但同时检测基因突变和肝癌免疫微环境的研究目前较少，本研究报道了 1 例肝细胞和 1 例胆管细胞癌相关检测结果，并根据本研究结果对未来的肝癌治疗提出初步见解。方法 在本研究中采用多重荧光免疫组化染色的方法，对 2 例患有肝癌的患者肿瘤组织进行检测，并实行肿瘤基因测序。后续对患者进行随访观察。结果 2 例患者均属于免疫无反应型。总体免疫检测结果显示对于单药免疫治疗的效果可能并不理想。部分基因检测结果也提示2 例患者可能对于免疫检查点抑制剂的治疗可能有效。但 2 例患者均未使用免疫治疗。结论 这 2 例患者的检测结果提示对于免疫治疗的效果并不理想。但对于肝癌而言，免疫治疗是目前极具希望的治疗方法，联合免疫治疗对肝癌可能具有一定的帮助。 

关键词:

肿瘤微环境 , 免疫检查点 , 免疫治疗 , 基因检测 , 联合免疫治疗