Objective To investigate the efficacy of clustering care program for heart transplant patients. Methods Twenty five heart transplant patients admitted to our hospital from June 2015 to December 2017 were enrolled as the control group，38 heart transplant patients admitted to our hospital from January 2018 to April 2019 were selected for observation group. The control group received the routine nursing method of heart transplantation. The observation group received the evidence-based clustering nursing program to implement the heart transplantation bundled nursing training，the nurses mastered the bundled nursing program and implemented it accurately. The postoperative mechanical ventilation time，intensive care unit stay time，hospitalization time， infection rate and incidence of severe rejection were compared between the two groups. The effect of cluster nursing on heart transplantation patients was analyzed. Results Compare to the control group，there were significant differences on the postoperative mechanical ventilation time（9.2±6.43）h vs.（14.92±12.44）h，the intensive care unit stay time（4.26±1.71）d vs.（6.21±1.19）d and the hospitalization time（18.05±4.1）d vs.（20.79±7.03）d， respectively（P ＜ 0.01）. While there were no differences in the incidence of infection and the incidence of severe rejection of the two groups. Conclusion For the nursing management of heart transplant patients，the application of clustered nursing intervention strategy can shorten the time of mechanical ventilation，intensive care unit stay time and hospitalization time.