Objective To detect the functional significance of surface inhibitory receptor leukocyteassociated immunoglobulin like receptor-1（LAIR-1）of NK cells in xenogeneic immune rejection. Methods The infiltration of immune cells was detected by the immunohistochemical staining in the donor liver tissue after xenotransplantation. The soluble LAIR-1（sLAIR-1）in the recipient plasma and membrane LAIR-1（mLAIR-1）in recipient immune cells were detected by the enzyme linked immunosorbent assay（ELISA）and RNA sequencing， respectively. Results In tissue level, the donor liver was infiltrated of lymphocytes. In molecular level, the sLAIR-1 and mLAIR-1 were in equilibrium. Conclusion The mLAIR-1of NK cell and sLAIR-1 in plasma were in balance and related to xenogeneic immune rejection. Taken all together these data suggest that the LAIR-1 represents an important target in inducing immunological tolerance.