Objective To establish an effective method for the extraction and culture of vigorous primary porcine hepatocytes and aortic endothelial cells, and provide the basis for the research of porcine vascular endothelial cells and hepatocytes which are the target cells in xenograft rejection. Methods Liver and aortic blood vessel were isolated from wild Bama pigs, followed by primary porcine hepatocytes extraction using peristaltic pump perfusion and type Ⅱ collagenase for digestion. Then porcine hepatocytes were purified by low speed centrifugation and differential adherence methods. Primary hepatocytes were identified by PAS staining, immunofluorescence staining for albumin and hepatocyte nuclear factor 4α. Endothelial cells of porcine aorta were extracted by type I collagenase digestion, and authenticated by testing factor Ⅷ associated antigen vWF and endocytosis of acetylated DiI-Ac-LDL. Results A large number of highly viable primary porcine hepatocytes and aortic endothelial cells were extracted, and they can express hepatocyte and endothelial cell marker proteins respectively. Conclusion The primary pig endothelial cells and hepatocyte extraction methods provided in this study are reliable methods for preparing a large number of highly viable primary endothelial cells and hepatocytes.