实用器官移植电子杂志 ›› 2018, Vol. 6 ›› Issue (5): 359-362.DOI: 10.3969/j.issn.2095-5332.2018.05.005

• 论著 • 上一篇    下一篇

抑制性分子 LAIR-1 在异种移植物杀伤机制中的作用

张虹 1,张卓超 2,李霄 1,黄敏 1,张玄 1,陶开山 1,窦科峰 1   

  • 出版日期:2018-09-20 发布日期:2021-06-24

The role of inhibitory molecule LAIR-1 in the killing mechanism of xenografts

  • Online:2018-09-20 Published:2021-06-24

摘要:

目的 探讨 NK 细胞表面抑制性受体白细胞相关免疫球蛋白样受体 -1(eukocyte-associated immunoglobulin like receptor-1,LAIR-1)在异种移植免疫排斥反应中的作用。方法 采用免疫组织化学 染色检测异种移植术后供肝组织的免疫细胞浸润情况,酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测受体血浆中可溶性 LAIR-1(sLAIR-1)的表达,转录组测序检测受体免疫细胞表面 膜型 LAIR-1(mLAIR-1)的表达。结果 异种移植术后供肝组织受到淋巴细胞浸润,受体淋巴细胞表面 mLAIR-1 和 sLAIR-1 呈动态平衡状态。结论 受体免疫细胞表面 mLAIR-1 和血浆中 sLAIR-1 的表达水平 处于动态平衡的状态可能与受体发生免疫排斥反应相关,LAIR-1 有望成为移植术后诱导免疫耐受的分子。

Abstract:

Objective To detect the functional significance of surface inhibitory receptor leukocyteassociated immunoglobulin like receptor-1(LAIR-1)of NK cells in xenogeneic immune rejection. Methods The infiltration of immune cells was detected by the immunohistochemical staining in the donor liver tissue after xenotransplantation. The soluble LAIR-1(sLAIR-1)in the recipient plasma and membrane LAIR-1(mLAIR-1)in recipient immune cells were detected by the enzyme linked immunosorbent assay(ELISA)and RNA sequencing, respectively. Results In tissue level, the donor liver was infiltrated of lymphocytes. In molecular level, the sLAIR-1 and mLAIR-1 were in equilibrium. Conclusion The mLAIR-1of NK cell and sLAIR-1 in plasma were in balance and related to xenogeneic immune rejection. Taken all together these data suggest that the LAIR-1 represents an important target in inducing immunological tolerance.