关键词:

长期常温机械灌注 , 离体肝脏保存 , 器官功能维护 , 器官医学

Abstract:

Objective To establish an ex situ machine perfusion（MP）system for long-term liverperfusion using normothermic machine perfusion（NMP）technology. Methods A porcine liver was connectedto the Life-X100 perfusion system for normothermic machine perfusion. Perfusion parameters and perfusate blood-gas analysis results were continuously monitored during the perfusion process to assess the ex situ liverfunction. Results The porcine liver was successfully perfused ex situ for 138 hours，with stable perfusionparameters and well-maintained liver function. Conclusion We have preliminarily established an ex situ longterm liver perfusion system. This system can be used for the ex situ maintenance of human livers in the future and can serve as a basis for various exploratory studies to advance medical development in our country. 

Key words:

Long-term normothermic machine perfusion , Preservation of liver in vitro , Organ function maintenance, Organ medicine