Abstract:

Objective To investigate the value of hypothermic continuous perfusion machine（LifePort） on the preservation and evaluation of grafts from expanded criteria donors（ECD）for renal transplantation and the value of the decision for transplantion prognosis. Methods The study included kidneys were procured from 52 ECDs underwent preserved with LifePort in First Hospital of Jilin University. The LifePort perfusion parameters and recipients' clinical data were analyzed retrospectively，such as delayed graft function（DGF）incidence，acute rejection rates，serum creatinine，1-year patient and kidney allograft survival rates. Results Among 104 ECDs kidneys underwent preserved with LifePort，8 kidneys were discarded after evaluation，and 96 kidneys were transplanted with no primary non-function（PNF）. The incidence of acute rejection（AR）was 9.38%（9/96），all AR cases were recovered with anti-rejection treatment. The DGF incidence rate was 17.71%（17/96），all DGF cases were recovered within one month. The average final resistance of the graft in patients who experienced DGF was significantly increased than that in patients with no DGF（P ＜ 0.05）. Inversely，the average final flow rate of the graft in patients with DGF was significantly decreased than that in patients without DGF（P＜0.05）. The follow-up period was rangedfrom 6 to 42 months（the median time was 15 months），the average serum creatinine of all recipients was（113.7± 38.4）μmol/L at 6-months follow-up， 1-year patient and kidney allograft survival rates were 100%. Conclusion LifePort played pivotal role in the preservation and evaluation of ECD grafts in renal transplants， combined with the LifePort perfusion parameters，the system gave assistance in any employ the graft or not，and predicted recipients' prognosis.