Objective To perform comprehensive preoperative testing in order to reduce the risk of hyperacute rejection（HAR）and acute rejection（AR）in the Wuzhishan miniature pig-Tibetan monkey xenotransplantation. To Minimize the influence of immunological factors on recipients survival in order to prolong the recipients survival. Methods The donor GGTA1 gene knockout type was identified by PCR and lectin cell staining，respectively. A total number of 30 Tibetan monkey and Wuzhishan miniature pigs were tested for lymphocyte toxicity and ABO blood group identification. Results The donor was GGTA1 knockout pigs，and the fluorescence was not detected in GTKO pig peripheral blood mononuclear cell（PBMC）with lectin cell staining，it indicated that the GGTA1 gene was successfully knocked out，which was consistent with the genotype identification results. The Tibetan monarch monkey 28# with the least toxicity to the donor PBMC through lymphocyte toxicity test was selected as a candidate recipient. Blood type test showed identical blood type between donor and recipients. Conclusion The preoperative test of GTKO Wuzhishan miniature pigs-Tibetan monkey xenotransplantation has greatly reduced the risk of HAR and AR after xenotransplantation.