Objective To investigate the causes of coagulation dysfunction in orthotopic liver transplantation by observing the results of thrombelastography（TEG） heparinase test. Methods There were 24 cases of patients undergoing orthotopic liver transplantation, including 20 males and 4 females. The age of included patients was 41 ~ 60 years old， and weight was 48 ~ 86 kg. All cases received orthotopic liver transplantation without bypass. The total amount of red blood cells and plasma used in the operation were recorded. The central venous blood sample was collected at the 15th min in the new liver stage and tested by the TEG analyzer. The blood samples were added to the TEG heparinase test cup and TEG normal test cup. If the two kinds of TEG did not coincide and the difference was significant， the protamine was given， and then the TEG tests were repeated. Results All the patients received a stable operation. At the 15th min in the new liver stage， the R， K，αangle and MA in TEG heparinase test cup group were significant difference compared to the normal test cup group（P ＜ 0.05）. After the protamine was given, results of tests suggested that there was no significant difference between the TEG heparinase test cup group and TEG normal test cup group in R， K，αangle and MA（P ＞ 0.05） Conclusion The anticoagulant effect of heparin is th e main factor of early coagulation dysfunction in new liver stage.