Objective To summarize the surgical experience of liver transplantation in children receiving left lateral lobe. Methods A total number of 26 patients who suffered end stage liver disease had undergone partial liver transplant from September，2014 to August，2015，data were collected and analyzed，who were donated by left lateral lobe of liver. Among all the cases，22 children patients received living donor liver transplantation， the others received "split liver transplantation". Results Neither mortality nor serious complications occurred in the living organ donors. One child died in perioperativeperiod. Other recipients showed no discomfort in long term follow-up. Conclusion With high quality operation，pre-operate assessment，left lateral lobe transplantation is fairly feasible.

Objective To summarize the experience of organ procurement and bench surgery from donation after citizen's death（DCD）. Method Analyze the clinical data of 47 pediatric DCD organ procurement and preparation from January 2013 to June 2016，and sum up the experience. Results A total number of 47 pediatric organs were procured，from which 34 livers and 46 kidneys were transplanted to 115 recipients. Eleven of 46 kidney donors were transplanted as en-bloc donors. Conclusion The risk of organ transplantation can be obviously reduced by regularly summarize the experience of organ procurement and preparation from pediatric DCD.

Objective To investigate the level of serum hemeoxygenase-1（HO-1）in biliary atresia （BA）and its clinical significance. Methods We selected 20 cases children suffered from BA which treated with pediatric living donor liver transplantation from October 1，2015 to December 31，2015，vein blood was collected at one week before operation，and measured the HO-1 expression by enzyme-linked immunosorbent assay（ELISA）. Other clinical indicators were recorded at the same time. Five normal children were selected as control group. Results The expression of HO-1 in patients were increased compared to the healthy children groups before operation（P ＜ 0.001）. Direct bilirubin was positively correlated with HO-1（R ＝ 0.576，P ＝ 0.008）. Conclusion The expression of HO-1 in children with BA was related to the degree of biliary obstruction.

Objective To explore the impact of hypothermic machine perfusion（HMP） throughmodified UW solution in liver transplantation and the value of assessing the quality of liver. Methods Reteplase（rPA）was added in the process of HMP to record the changes of pressure and flow of hepatic artery and portalvein. We performed needle biopsy to liver before and after perfusion. The recovery of liver function after transplantation was observed. Results The pressure of hepatic artery and portal vein appeared descendingtrend and the flow appeard ascending trend，which maintained stable for a period of time. After adding rPA，the pressure kept declining while the flow kept rising. The perfusion liver function was better after livertransplantation. Conclusion The use of modified UW solution in HMP had clear safety and efficacy in clinic.It could reduce the vessell resistance in perfusion，contribute to the recovery of liver and assess the value of liver.

Objective To investigate the early changes of serum globulin and the risk factors influence to the reduction of serum globulin，and analyze its impact on short-term incidence of acute rejection（AR）and prognosis for patients after liver transplantation（LT）. Methods A total number of 170 patients who underwentcadaveric donor LT from Jan-Dec 2011 in our hospital were followed and the data has been statistical processing，median reduction level（D）of postoperative day（POD）1 compared with pre-operation was used as the cut off，85 patients in D ≥ 10.5 g/L group，85 patients in D ＜ 10.5 g/L group. The general information，surgical data，postoperative rejection and living conditions in recipients were retrospectively collected，and the risk factors that influence the recipients post operation globulin level and 1-year, 3-years cumulative survival rate were analyzed. Results As compared with pre-operative globulin，two groups decreased significantly on POD 1，then gradually increased. The POD 1 globulin level of D ≥ 10.5 g/L group was higher than that of D ＜ 10.5 g/L group（P ＜ 0.05）， there was no statistical significance at other time points（P ＞ 0.05）. Compared with D＜ 10.5 g/L group，patients in D≥ 10.5 g/L group had a lower weight〔weight（kg）：68.32±12.31 vs. 72.12±11.50，P ＜ 0.05〕；and graft to recipient weight radio（GRWR），cold ischemia time，the time of anhepatic phase，intraoperative blood loss in D ≥ 10.5 g/L group were higher than which in D ＜ 10.5 g/L group〔GRWR（%）：0.81±0.23 vs. 0.74±0.20，cold ischemia time（min）：9.86±2.66 vs. 8.85±2.45，the time of anhepatic phase（min）：48.95±19.59 vs. 44.11±9.35，intraoperative blood loss（L）：2.73±1.58 vs. 2.08±1.70，P ＜ 0.05〕；and incidence of acute rejection was higher too（P ＜ 0.05）. Logistic regression analysis showed that cold ischemia time and intraoperative blood loss were independent prognostic factors for D ≥ 10.5 g/L after LT. 1-year，3-year cumulative survival rate of 170 recipients was 88.4% and 77.6%，1-year，3-year cumulative survival rate in D ≥ 10.5 g/L group was 86.1% and 77.1%，and that in D ＜ 10.5 g/L was 90.8% and 78.4%（P ＞ 0.05）. Conclusion The early reduction of serum globulin after LT was inevitable and reversible process，and the reduction degree of globulin was associated with cold ischemia time and intraoperative blood loss，and the high reduction degree group had higherincidence of acute rejection，however the reduction degree had no impact on survival prognosis for patients after LT.