Abstract:

Objective To explore the impact of hypothermic machine perfusion（HMP） throughmodified UW solution in liver transplantation and the value of assessing the quality of liver. Methods Reteplase（rPA）was added in the process of HMP to record the changes of pressure and flow of hepatic artery and portalvein. We performed needle biopsy to liver before and after perfusion. The recovery of liver function after transplantation was observed. Results The pressure of hepatic artery and portal vein appeared descendingtrend and the flow appeard ascending trend，which maintained stable for a period of time. After adding rPA，the pressure kept declining while the flow kept rising. The perfusion liver function was better after livertransplantation. Conclusion The use of modified UW solution in HMP had clear safety and efficacy in clinic.It could reduce the vessell resistance in perfusion，contribute to the recovery of liver and assess the value of liver.

摘要：

目的 探索改良后的灌注液经机械灌注对供肝质量改善的影响以及在评估供肝质量方面的价值。方法 在低温机械灌注（hypothermic machine perfusion，HMP）过程中添加重组人组织型纤溶酶原激酶衍生物（reteplase，rPA），记录灌注过程中肝动脉和门静脉压力、流量的变化，对灌注前后的肝脏进行穿刺活检，观察灌注后供肝肝移植后的肝功能恢复情况。结果 肝动脉、门静脉压力、流量随着灌注的进行分别呈现下降和上升的趋势，一段时间后维持在稳定水平，添加 rPA 后动脉压力继续下降，流量继续增加。灌注供肝移植后肝功能恢复较好。结论 使用改良后的灌注液对供肝机械灌注，在临床上有明确的安全性和有效性，可以降低灌注过程中血管阻力，有助于供肝的修复，同时也可以用于初步评估供肝质量。