Objective The purpose of this study was to investigate the attitudes trend and characteristicsof family members of potential donation after cardiac death（DCD）donors in China. Methods This largest single center cohort study was performed by an interview or by telephone using a questionnaire. The family members of potential DCD donors were recruited from the first affiliated hospital，medical college of Xi'an Jiaotong universitylocated in a metropolitan area of northwest of China. The 12-item attitude questionnaire was specifically developed from the literature review and coordinator，physician，and donor's family feedback. The participants were asked to ratethe queries on a 5-point Likert intensity scale. Results The 174 participants included 56（32.2%）women and 118 （67.8%）men. Most of people were aged between 41 and 50 years old（n ＝ 63，36.2%），31 and 40 years old（n ＝59，33.9%），and less than 30 years old（n ＝ 36，20.7%）. The top five attitudes of participants were the best personto suggest organ donation to a family was ranked as the DCD coordinator of Red Cross Organization（RCO）（n ＝ 160， 92%），donor was a hero（n ＝ 143，82.2%），honor to be a donor's family member（n ＝ 136，78.2%），improvedrelationship with colleagues（n ＝ 124，71.3%），and with recipient after donation（n ＝ 123 70.7%）. The best personto suggest organ donation to a family was ranked as the coordinator of RCO（n ＝ 160，92%），doctor unrelated to transplantation（n ＝ 104，59.8%），social worker（n ＝ 36，20.7%），and doctor related to transplantation（n ＝ 25，14.4%）. The top two reasons for non-consent to donation were the family insisted on intact body after patient death， and did not want to have surgery again（n ＝ 51，41.5%），and feared that they would be misunderstood by neighbors， relatives and friends about donation（n ＝ 28，22.8%）. Conclusion This study revealed initial attitudes towardDCD organ donation in China. Some data afford insight to the decision-making procedure. The concerns of potential DCD donors and their families may help professionals provide better interventions in the future.

Objective To investigate the significance of color doppler ultrasound for detecting hepatic arteryin pediatric liver transplantation. Methods Application of color doppler ultrasound to observe the hepatic artery in57 pediatric after liver transplantation，observe whether the signal of blood flow，systolicvelocity（S），diastolic velocity （D）and S/D. The minimum follow-up time is 2 months，the longest is 9 months. All patients with artery detection data were collected for analysis. Results No obvious abnormality of hepatic artery was observed in 52 cases with colordoppler ultrasound. Arterial flow signal could not be detected in 5 patients 5-8 days after transplantation and hepaticartery thrombosis was considered，in which 3 cases were confirmed by digital subtraction angiography（DSA），1 case by contrast-enhanced ultrasound and CT，and 1 case was confirmed to be ultrasonic false positive with DSA showedno obvious abnormal hepatic artery. Diagnosis coincidence rate of color doppler ultrasound in hepatic artery detection was 98%. Conclusion Color Doppler ultrasound provides an important basis for hepatic artery monitoring and early diagnosis of complication after liver transplantation.

Objective To compare the accuracy of radiologically estimated liver volume by computedtomography（CT）and magnetic resonance imaging（MRI）. Methods Cochrane library，abstract databases 〔Medline，Embase，China biology medicine disc（CMBdisc）〕and full text databases（Springer，OVID， Sciencedirect，Ebsco，CNKI，WanFang and VIP）were searched for published papers on whole liver volumetrywith CT or MRI modality. Inclusion criteria were based on validity criteria for diagnostic research published by theCochrane collaboration. Eligible papers were grouped according to imaging modality（CT or MRI）and evaluated forquality before data extraction. With meta-analysis package Meta of R software，the statistical model used to pool the

result was selected according to result of heterogeneity test. Group-wise meta-analysis and comparison of included studies of CT and MRI liver volumetry were performed. The pooled result was weighted mean difference between estimated and true liver volume. Finally sensitivity and publish bias analysis was performed. Results 17 articles with 37 studies including 351 patients meet the inclusion criteria. There was no heterogeneity among included studiesof each group（CT ：I2 ＝ 0%，Q ＝ 4.93，P ＝ 0.999 8 ；MRI ：I2 ＝ 0%，Q ＝ 0.92，P ＝ 1）. Fixed effect modelwas selected for the meta-analysis and the weighted mean difference was 21.706 3 ml〔95％ confidence interval （95%CI）＝ -8.015 4 - 51.464 0 ml）〕for the CT group and 20.951 8 ml（95％ CI ＝ -60.436 0 - 102.339 5 ml）for the MRI group. There was no statistically significant difference between the true and radiologically estimated whole liver volume of each group（CT ：Z ＝ 1.429 7，P ＝ 0.152 8 ；MRI ：Z ＝ 0.504 6，P ＝ 0.613 9）and between pooled results of each group（Q ＝ 0，P ＝ 1）. Sensitivity analysis revealed the pooled result of each group was robust which was not affected by excluding any of the included studies or changing the effect model. Funnel plot and publish bias analysis revealed no publish bias（CT ：t ＝ -1.882 0，P ＝ 0.075 2 ；MRI ：t ＝ 0.001 5，P ＝ 0.998 9）. Conclusion Accurate liver volumetry could be performed with CT or MRI images. The imaging acquisitionmethods should be selected according to the patients' general status with respect to the patients' benefits.

Objective To evaluate the left and right ventricular function by Tei index assessed by pulsed wave doppler imaging in patients with liver cirrhosis before orthotopic liver transplantation（OLT）. Methods InTianjin First Center Hospital，between May 2014 and September 2014，we choose 38 patients with liver cirrhosisbefore OLT as cirrhosis group，27 healthy controls as control group and let them underwent color doppler echocardiography. The peak filling velocity of E wave and A wave，ejection fraction（EF）of left and right ventricular were measured，E/A and Tei index were calculated and compared. Results There was no significant difference in left ventricular end diastolic diameter（LVDd）、left atrial end systolic diameter（LADs）、right ventricularend diastolic diameter（RVDd）、right atrial end systolic diameter（RADs）、EF of left and right ventricular andE/A（all P ＞ 0.05）between cirrhosis group and control group. Tei indices of left and right ventricle in cirrhosis group（0.56±0.12，0.46±0.10）were higher than those of normal control group（0.41±0.07，0.37±0.06）（both P ＜ 0.05）. Conclusion Tei index assessed by pulsed wave Doppler imaging is a good way to evaluate the global heart function. It can be used to evaluate the patients with liver cirrhosis before OLT and provide guidance for clinical treatment.

Objective To investigate the etiology，diagnosis and treatment of graft-versus-host diseaseafter liver transplantation. Methods Progression，histology，diagnosis and treatment of one suspected case of GVHD after liver transplantation were analyzed and correlative literatures were reviewed. Results The patient suffered fever，epilepsy，rashes and hematopoietic stagnation 2 weeks after liver transplantation and had no response to anti-infection therapy. Considering of clinical symptoms together with the histological appearance of skin lesions，blood and bone marrow cytology tests，and T-cell subsets，GVHD was suspected. Immunosuppressants were discontinued and high-dose steroid therapy with 1 000 mg/day intravenous methylprednisolone was administered. Skin rashes faded，but the general condition deteriorated and the patient died of multiple organ failure 3 weeks afterliver transplantation Conclusion GVHD is a rare and life threatening complication after liver transplantation withmortality ＞ 75%. There is no standard diagnosis criterion. We summarize this suspected case and review literatures about GVHD after liver transplantation in order to increase awareness of the disease.