Abstract:

Objective To investigate the level of serum hemeoxygenase-1（HO-1）in biliary atresia （BA）and its clinical significance. Methods We selected 20 cases children suffered from BA which treated with pediatric living donor liver transplantation from October 1，2015 to December 31，2015，vein blood was collected at one week before operation，and measured the HO-1 expression by enzyme-linked immunosorbent assay（ELISA）. Other clinical indicators were recorded at the same time. Five normal children were selected as control group. Results The expression of HO-1 in patients were increased compared to the healthy children groups before operation（P ＜ 0.001）. Direct bilirubin was positively correlated with HO-1（R ＝ 0.576，P ＝ 0.008）. Conclusion The expression of HO-1 in children with BA was related to the degree of biliary obstruction.

摘要：

目的 探讨胆道闭锁（biliary atresia，BA）患儿血清血红素氧化酶 -1（hemeoxygenase-1， HO-1）表达水平及其临床意义。方法 连续收集 2015 年 10 月 1 日 -2015 年 12 月 31 日间接受亲属活 体供肝移植的 BA 患儿 20 例，于术前 1 周采集受者外周静脉血，采用酶联免疫吸附试验（enzyme-linked immunosorbent assay，ELISA）检测血中 HO-1 的表达量。同时记录相关临床指标。选取 5 例健康同龄儿童 作为对照组。结果 BA 患儿血清 HO-1 测定值高于对照组（P ＜ 0.001）。直接胆红素与 HO-1 呈正相关 （相关系数 R ＝ 0.576，P ＝ 0.008）。结论 BA 患儿血清 HO-1 值升高与胆道梗阻程度相关。