Practical Journal of Organ Transplantation(Electronic Version) ›› 2014, Vol. 2 ›› Issue (4): 222-229.DOI: 10.3969/j.issn.2095-5332.2014.04.005

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Intracellular adenosine-tri-phosphate levels arelower in pediatric liver transplant recipients withinfection

Rao Wei1,2,Xie Man3 ,Sun Xiaoye1,2,Yang Tao1,2,Gao Wei1,2,Zhang Jianjun1,2,Liu Yihe1,2,Deng Yonglin1,2,Shen Zhongyang1
  

  1. 1. Department of Organ Transplantation,First Center Hospital,Tianjin 300192,China ; 2. TianjinKey Laboratory of Organ Transplantation,Tianjin 300192,China ; 3. Department of Digestive Diseases,First CenterHospital,Tianjin 300192,China
  • Online:2014-07-20 Published:2021-04-29
  • Supported by:
    Supported by Fund of National High-tech Researching and Developing Plan(Plan863)(2012AA021001)

Intracellular adenosine-tri-phosphate levels arelower in pediatric liver transplant recipients withinfection

Rao Wei1,2,Xie Man3 ,Sun Xiaoye1,2,Yang Tao1,2,Gao Wei1,2,Zhang Jianjun1,2,Liu Yihe1,2,Deng Yonglin1,2,Shen Zhongyang1   

Abstract:

ObjectiveToanalyzeintracellularadenosine-tri-phosphate(iATP)levelsinpediatricliver transplant(PLT)recipients andto investigateitscorrelationwithpatients'clinicalstatus.MethodsSixtythreePLTpatientswererecruitedtothisstudy.Patientsweredividedintoclinicallystableadinfectiongroups.AssociationofiATPlevelswithepisodesofinfectionwas analyzed.ResultsAmong 63patients,120iATPvalues wereobtainedincludingserial iATPmeasurementsfrom35patients(55.6%).TheaverageiATPvalueof the 64samples from 39clinicallystablechildrenwas(372±225)μg/L.Highinter-individual iATPvariabilitywasobservedin clinicallystablepatientsandiATPlevelsappeareldstableinindividuapatientswithserialdata.TwentyfourPLTrecipientswithinfectionhadsignificantlyloweriATPattimeofinfectioncomparedwithclinicallystablerecipients(P<0.001).Serial measurementsindicatedthattheaverageiATPvaluedecreasedsignificantly asinfectionprogressed(P<0.001),and increasedas infectionresolved(P=0.036).However,12of the39clinicallystablepatientswith lowiATPvalues(iATP<225μg/L)showednoinvasiveinfection,while8ofthe24infectedpatients showedhighlevelsof iATPduringinfection(iATP>225μg/L). ConclusionsComparedwithclinicallystablepatientsloweriATPlevelswereobservedininfectedPLTrecipients.Levelsbecameelevatedasinfectionresolved.TheinterindividualvariabilityobservedinourstudyindicatesthatcutoffvaluesofiATPmaynotbesuitablefordefiningimmunestatus. Serial monitoring datamaybe more helpful todetermine the cellular immune response in PLT recipients.

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摘要:

目的 对儿童肝移植受者中 CD4+ T 淋巴细胞内三磷酸腺苷(iATP)含量(ImmuKnow 法)进行分析,并探讨其与受者临床状态的关系。方法 本研究共纳入 63 例儿童肝移植受者,将其分为临床稳定组和感染组,分析 CD4+ T 淋巴细胞内 iATP 含量与感染的关系。结果 63 例患儿中,共有 35 例(55.6%)患儿进行了 iATP 的系统检测,共计 120 例次。其中,39 例临床稳定组患儿的 64 例次 iATP 的平均值为(372±225)μg/L,且个体差异性较大。与临床稳定组患儿相比,24例感染组儿童受者的iATP更低,差异具有统计学意义(P < 0.001)。系统监测显示,随着感染进展,iATP水平均值显著下降(P<0.001),且随着感染好转,其水平又有所升高(P=0.036)。另外,12例iATP水平偏低(iATP<225μg/L)的临床稳定组患儿中,并未发现活动性感染,而 8 例感染组患儿中,其 iATP 水平却偏高(iATP > 225 μg/L)。结论 与临床稳定组的患儿相比,感染组患者的 iATP 水平更低,且随着感染好转,其水平逐渐升高。本研究发现,iATP 水平的个体差异性较大,提示 iATP 水平检测可能只是反映患儿免疫状态的一个指标。iATP 水
平的系统监测将更有助于提示儿童肝移植受者免疫状态变化。

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