Abstract:

Objective To investigate the expression and scientific significance of PBRM1 in renal transplantation ischemia reperfusion injury（IRI）. Methods Three groups of kidney tissues（5 cases in eachgroup）were selected and divided into normal kidney group（Control），stable allograft function（STA group），and ischemia injury（IRI group）. The expression of polybromo 1（PBRM1）in the three groups was detected by immunohistochemistry. The expression of PBRM1 after renal IRI was analyzed with Gene Expression Omnibus（GEO）data set and its mechanism was discussed. Finally，the expression of Pbrm1 in Th17 cells was verified in vitro. Results Immunohistochemical staining showed that the expression of PBRM1 in IRI group was significantly higher than that in the other two groups. GSE180420 database analysis showed that PBRM1 expression was significantly increased in IRI group compared with Control，and GSEA results suggested that PBRM1 could promote the function of Th17 cells. In vitro experiments confirmed that the transcription level of Pbrm1 was significantly increased in Th17cells compared with Th0 cells. Conclusion The expression of PBRM1 was significantly increased after renal IRI transplantation. PBRM1 may aggravate renal IRI by affecting the function of Th17 cells. 

Key words:

 , Kidney transplantation； Ischemia reperfusion injury； PBRM1； Th17 cell

摘要：

目的 探讨 PBRM1 分子在移植肾缺血 / 再灌注损伤（ischemia reperfusion injury，IRI）中的表达及其作用机制。方法 选取 3 组肾脏组织（每组 5 例），分为正常肾脏组（Control 组）、移植术后肾功能稳定组（STA 组）、移植肾缺血 / 再灌注损伤组（IRI 组）。采用免疫组化技术检测多溴蛋白 1（polybromo 1， PBRM1）在 3 组中的蛋白表达。结合基因表达数据库（Gene Expression Omnibus，GEO）数据集分析 PBRM1 在肾脏 IRI 后的表达，探讨其机制，并通过体外实验验证 Pbrm1 分子在 Th17 细胞的表达。结果 免疫组化 染色结果显示，与 Control 组和 STA 组相比，IRI 组的 PBRM1 表达程度显著高于其他两组。经 GSE180420 数据库分析显示，与 Control 相比，IRI 组 PBRM1 分子表达水平显著升高，GSEA 结果提示 PBRM1 可促进Th17 细胞的功能。体外实验证实，与 Th0 细胞相比，Pbrm1 基因的转录水平在 Th17 细胞中显著升高。结论 移植肾 IRI 后 PBRM1 分子表达水平显著升高，PBRM1 分子可能通过影响 Th17 细胞的功能，从而加重肾脏 IRI。 

关键词:

肾移植 ； 缺血 / 再灌注损伤 ； 多溴蛋白 1 ； Th17 细胞 ,