ObjectiveToanalyzeintracellularadenosine-tri-phosphate（iATP）levelsinpediatricliver transplant（PLT）recipients andto investigateitscorrelationwithpatients'clinicalstatus.MethodsSixtythreePLTpatientswererecruitedtothisstudy.Patientsweredividedintoclinicallystableadinfectiongroups.AssociationofiATPlevelswithepisodesofinfectionwas analyzed.ResultsAmong 63patients，120iATPvalues wereobtainedincludingserial iATPmeasurementsfrom35patients（55.6%）.TheaverageiATPvalueof the 64samples from 39clinicallystablechildrenwas（372±225）μg/L.Highinter-individual iATPvariabilitywasobservedin clinicallystablepatientsandiATPlevelsappeareldstableinindividuapatientswithserialdata.TwentyfourPLTrecipientswithinfectionhadsignificantlyloweriATPattimeofinfectioncomparedwithclinicallystablerecipients（P＜0.001）.Serial measurementsindicatedthattheaverageiATPvaluedecreasedsignificantly asinfectionprogressed（P＜0.001），and increasedas infectionresolved（P＝0.036）.However，12of the39clinicallystablepatientswith lowiATPvalues（iATP＜225μg/L）showednoinvasiveinfection，while8ofthe24infectedpatients showedhighlevelsof iATPduringinfection（iATP＞225μg/L）. ConclusionsComparedwithclinicallystablepatientsloweriATPlevelswereobservedininfectedPLTrecipients.Levelsbecameelevatedasinfectionresolved.TheinterindividualvariabilityobservedinourstudyindicatesthatcutoffvaluesofiATPmaynotbesuitablefordefiningimmunestatus. Serial monitoring datamaybe more helpful todetermine the cellular immune response in PLT recipients.

Objective To retrospectively analyze the incidence and risk factors of acute respiratorydistress syndrome（ARDS）in post-liver transplant adult patients, and to approach the effect of bilirubin onmorbidity and prognosis of ARDS. Methods After winninginstitutional review board approval，clinicaldata of adult patients（age ≥ 18 years）undergone liver transplantation from UCLA Transplant Database Centerbetween January 1st，2004 and December 31st，2011 were collected. Diagnosis of ARDS was determinedusing criteria proposed by the 2011 Berlin definition. All patients were underwent general anesthesia，andthey were divided into ARDS group and non-ARDS group（No-ARDS）. The general state and laboratoryexamination results including total bilirubin （TBil），creatinine （Cr），electrolyte （K+，Na+），and prognosiswere analyzed. Statistical analyses were performed using the IBM SPSS 20.0. Results Of 1 335 patientsundergone liver transplantation，57 cases（4.3%）occurred postoperative ARDS. Univariate analysis showedARDS was associated with significantly higher preoperative model for end-stage liver disease（MELD）scoreand requirement of endotracheal intubation（both P ＜ 0.05）. TBil in the ARDS group were significantly higher

Objective To discussing the influence of normative postoperative nursing intervention onthe obedience of liver transplantation patient，extend the medical intervention to family and outside hospital，andreduce the transplantation organ damage caused by violated medical treatment. Methods Forty post-operationliver transplantation patients in follow-up center were randomly enrolled as the research objects. A detailedquestionnaire survey about impact factors of follow-up-such as age，income，education，transplant post-operativetime，individual patients' request were conducted. One-way ANOVA was used to conduct statistical analysis viaSPSS application. Results Different age，income，education，transplant post-operative time lead to differentrequests for follow-ups，and then affect the follow-up frequency and results which lead lower obedience. Theresults of living habitobedience and self-monitoring obedience of young patients and whose post-operation timenot less than one year was worse，and the results of self-paid patients was worse than those had health insurance（all P ＜ 0.05）. Conclusion Reasonable nursing intervention can improve the follow-up frequency and results.Postoperative survival rate and life quality can be improved by enhancing obedience through setting individual follow-up plan to different patients according to their own needs in different time.

Objective To understand and explore the cause of change in perioperative psychology inpatients undergone liver transplantation in order to put forward the corresponding nursing measures. Methods Sixtypatients undergone allograft orthotopic liver transplantation in the People's Liberation Army 302 Hospital betweenSeptember 2011 and February 2011 were enrolled. Through the way of clinical observation and communication，theperioperative psychological of liver transplant recipients was investigated with self-reported depression scale（SDS）. Thepreoperative and postoperative SDS score in recipients was compared，and the incidence of depression at different postoperative period was recorded. Results With prolongation of postoperative time，the SDS score of recipientswas gradually declined ；there was significant difference in SDS score between half a month of pre-operation and halfa month of post-operation（59.30±3.51 vs. 48.07±4.93，P ＜ 0.05）. After liver transplantation，the recipientswith depression at different period were gradually lowered as well as the incidence of depression（the incidence ofdepression postoperative 1-3 months，4-6 months，and 7-12 months was 31.7%，26.7%，20.0%，respectively）withoutstatistical difference（P ＞ 0.05）. Conclusion Reconstruction of psychological change in patients undergoneliver transplantation，taking effective mental dredge and targeted nursing can effectively avoid the occurrence of psychological problems，and improve the quality of survive.

Objective To evaluate the immunogenicity of the DNA ligand of DNA immunoadsorbent by observing the serum antibody production，lymphocyte proliferation and changes in immune organ and tissue in rabbits. Methods Twenty-four healthy rabbits were randomly divided into high-dose DNA group，low-dose DNAgroup，low-dose DNA + adjuvant group，and blank control group，with 6 rabbits in each group. Serum antibody titer was determined by the methods of double agar diffusion and enzyme linked immunosorbent assay（ELISA）. The liver and kidney tissues were collected for pathology observation. The spleen was collected to isolate the lymphocytes，which were divided into double strands DNA experiment group，nonspecific immunostimulator concanavalin（ConA）positive control group and cell culture fluid negative control group. The lymphocytes were cultured in vitro with the peripheral blood lymphocytes of rabbit. Spleen lymphocyte proliferation was observed by methylthiazolyl tetrazolium （MMT）colorimetry. Results The double agar diffusion results showed that double stranded DNA could not stimulate the immune system to produce antibodies in rabbits. ELISA results showed that double stranded DNA hadno immunogenicity，which could not stimulate the production of corresponding antibody（F＝1.183，P＝0.341）.The number of splenic lymphocyte proliferation（A value）in negative control group，DNA experiment group，and positive control group were 0.241±0.040，0.850±0.070，0.576±0.078，respectively，but there were no significantdifferences among three groups（F＝1.183，P＝0.336）. No pathological change and lymphadenosis was found byhistopathology results. Conclusions The DNA ligand of DNA immunoadsorbent cannot stimulate rabbits to producespecific antibodies，nor stimulate the lymphadenosis in immunized rabbits. The DNA ligand has no immunogenicity.