Abstract:

Objective To clear the applicable value of standardized protocols of sampling in organ transplantation which were established to obtain high quality samples. Methods From September 2014 to September 2016, samples in the Tianjin First Center Hospital biobank were studied, which were obtained and stored following the protocols of International Association of Environmental and Biological Samples（ISBER）and China Biosafety Society. Paraffin tumor sections from 200 patients who underwent liver transplant were studied. Tumor necrosis was assessed by HE staining. DNAs/RNAs were extracted from tumor tissues, which were quantified by gel electrophoresis. Results A total of 200 samples were included in this study. Tumors Area ≥ 80% was found in 192（96%）samples. The A260 / A280 of RNAs in tissues was 1.9-2.0. Gel electrophoreses showed that the 28S and 18S bands were clear and no tailing, indicating that the RNA integrity was good. The A260 / A280 of DNA in tissues was 1.8-1.9, and electrophoreses of the DNA showed clear and no obvious degradation. Conclusion Standardized collection, management and preservation for samples from organ transplantation may contribute to establish high quality sample resources for studies of this field.