Objective To summarize the experience of donor heart evaluation and function maintenance from 37 cases of donation after brain death in our hospital. Methods From January 2013 to June 2017，37 cases of heart donation after brain death were evaluated in cardial surgery department，Tianjin First Center Hospital. Donor hearts were fromorgan donation from organ procurement organization（OPO），and the diagnoses of brain death were based on the China brain death criteria（adult）. Results Transplantations were all performed with a double lumen venous anastomosis manner. The cold ischemia time was（45 ～ 370）min，aorta cross-clamping time was（55 ～ 110）min and cardiopulmonary bypass time was（125 ～ 190）min. The operations were successful in 36 patients. A cardiac dysfunction occurred after operation in 1 case whose body weight was 30% less than the donor. The patient recovered after treated with extracorporeal membrane oxygenation（ECMO），intra-aortic balloon pump（IABP）and continuous renal replacement therapy（CRRT）1 week later. Conclusion We can improve the utilization of donor hearts andthe long-term survival of heart transplantation recipients by a comprehensive evaluation of the donor and recipient