关键词:

荧光素酶 , 胰岛 , 裸鼠 , 移植

Abstract:

Objective To investigate the labeling of mouse pancreatic islets with lentivirus Luciference and to study their fluorescence expression in vitro and in vivo after transplantation in nude mice. Methods The purified and cultured ICR mouse islets were transfected with lentivirus-LUC islet culture medium with a multiplicity of infection of 10 MOI， the islets were then washed and cultured for 24 h. The fluorescence expression of islets labeled with different isletequivalents of LUC in vitro were observed by optical imaging system of small animals within 14 days of culture. In vivofluorescence expression was observed within 30 days after renal subcapsular transplantation in nude mice. Results The activity of islets after LUC gene transfection by this method was （86.7±6.7）%. Using IVIS Lumina LT， LUC-labeled isletsshowed fluorescent expression while cultured in vitro，and the amount of fluorescent expression was related to islet equivalent. In vitro，the fluorescence expression intensity showed a trend of increasing over time within 14 days of culture. After transplanted under the renal capsule of nude mice with LUC-labeled islets， long-term fluorescence expression was seen （＞ 30 days）. However， the intensity of fluorescence expression fluctuated greatly over time.Conclusion In this study，the survival of LUC-labeled islets after transplantation in vivo can be observed in vivo for a long time， which provides a method reference for the follow-up study on the survival and efficacy of islets at the transplant site. 

Key words:

 , Luciference； , Pancreatic islets； , Nude mice； , Transplantation