Objective To analyze the relationship between the energy metabolism of donor liver before and post recanalization of blood vessels and the ischemic injury of bile duct injury after liver transplantation. Methods The data of patients who received liver transplantation in Tianjin First Center Hospital between March 2014 and August 2015 were analysed retrospectively. The patients who were satisfied with the standard were enrolled，and samples were taken before recanalization of portal vein，before recanalization of hepatic artery and 1 hour after recanalization of hepatic artery. Patients were followed for one year，patients enrolled were divided into ischemic-type biliary lesions（ITBL）group and non-ischemic-type biliary lesions（NITBL）group according to results of cholangiography. ATP，ADP and AMP at three timepoints in two groups were compared. Results There was no significance difference in energy metabolism showed by indices such as ATP，ADP and AMP， between ITBL group and NITBL group before recanalization of portal vein, before recanalization of hepatic artery and1 hour after recanalization of hepatic artery（P ＞ 0.05）. Conclusion There was no relationship between energy metabolism of donor liver and the occurrence of ITBL post liver transplantation.