Abstract:

Objective The study the morphology and pathology of autologous blood cell from patients withtumor during liver transplantation. Methods Among 124 patients who underwent orthotopic liver transplantation，55 patients with tumor were enrolled. Intraoperative autologous blood recovery apparatus（Cell Saver 5 or 5+）was used to collect autologous blood cell. The red blood cell volume of fully automatic washing was 225 ml/cycle. The recycled autologous blood cell was detected by smear analysis and the pathologyof recycled autologous blood cell was observed. Results Among the 55 patients with preoperative tumor，36 patients didn't receive fully automaticwashing because of less blood lose. The other 19 patients' total volume of recycled autologous blood cell was 225 ml - 13 900 ml. There were 7 patients who didn't receive autologous blood cell infusion because of blood lose less than 1 000 ml. Among 12 patients whose blood lose more than 1 000 ml，6 cases whose recovery blood cell was much enough to receive fully automatic washing for more than 5 times. At last，there were 10 patients received autologousblood cell infusion while 2 patients were suspected tumor rupture during procedure didn't receive autologous bloodcell infusion. All the red blood cells showed normal morphology in blood sample smear analysis，and no tumor cells were found in pathological examination. Crushed neutrophils were found occasionally，lymphocytes can be observed rarely. Low power light microscope，liver cancer cells arranged in a trabecular，solid nests，pseudoglandular or acinar structures，cells arranged in three or four layers thick trabecular or cords. At high magnification，the size of the cancer cells varied，morphology of the cancer cells weird，abundant acidophilic cytoplasm，round andhyperchromatic nucleus，nucleolus clear，mononucleated or multinucleated，osteoclastic-like giant cells were observed，occasionally. Conclusion In the samples of recycled autologous red blood cell，no tumor cell was found in the aspects of blood cell morphology or tumor cell pathology. he autologous transfusion can be used for the patients whose blood lose more than 1 000 ml and rupture of the tumor did not during operation.