Objective To investigate the susceptibility profile of clinical isolates collected from renal transplant ward in a teaching hospital in 2013-2017. Methods Antimicrobial susceptibility testing was using Kirby-Bauer method or automated systems. Results were analyzed according to CLSI 2017 breakpoints. ResultsA total number of 7 530 clinical specimens were collected from renal transplant unit in 2013—2017，including urine 2 521（33.5%），drainage of liquid 2 299（30.5%），blood specimens 1 187（15.8%），other 1 523（20.2%）. A total number of 1 536 isolates，648 isolates not repeated，590 strains（91.0%）was bacteria，58 strains（9.0%）was fungi， the top five isolates were Enterococcus faecium（19.8%），Escherichia coli（15.6%），Klebsiella pneumoniae（8.3%）， Pseudomonas aeruginosa（4.6%）and Acinetobacter baumannii（4.5%），respectively. Separation of fungi is given priority to with Candida albicans and Candida glabrata（46.4%）. Prevalence of ESBLs-producing strains was 89.1% in Escherichia coli，71.7% in Klebsiella pneumoniae，8.2% of these strains were resistant to carbapenems. The resistant rate of 69.0% to Pseudomonas aeruginosa and 23.3% to Acinetobacter baumannii，respectively. The overall prevalence of methicillin-resistant strains was 78.6% in Staphylococcus aureus and 93.3% in Staphylococcus epidermidis， respectively. No staphylococcal strains were found resistant to vancomycin. The resistance rates of Enterococcus faecalis strains to most drugs tested（except tetracyclines）were much lower than those of Enterococcus faecium. No strains of both species were resistant to vancomycin Conclusion Bacterial resistance to commonly used antibiotics is still on the rise，especially the Carbapenem-resistant Enterobacteriaceae（CRE）isolates has a tendency to increase year by year. It is necessary to strengthen hospital infection control and management of clinical use of antimicrobial agents，and maintain good practice in surveillance of bacterial resistance.