Objective To discuss the clinical experience of liver and kidney procurement from donation after brain death（DBD）donors. Methods The clinical data of 12 DBD donors undergoing liver and kidney procurement in Tianjin First Central Hospital from May 2016 to March 2018 were analyzed retrospectively. The clinical experience was summarized. Results According to the diagnostic criteria for brain death established by Brain Injury Evaluation Quality Control Center of National Health and Family Planning Commission，the livers and kidneys of 12 DBD donors were procured after brain death．Modified liver and kidney perfusion was performed by arterial and portal vein intubation using the catheter made in Tianjin First Central Hospital．The operation time was 40 ～ 60 min．A total of 12 liver grafts and 24 renal grafts were harvested，those organs were successfully applied in clinical liver and renal transplantation．No primary nonfunction was observed in the liver or renal grafts. Conclusion Timely assessment of brain death，rigorous and cautious measurement for organ maintenance and modified techniques for combined liver and kidney procurement play a key roles in the success of liver and kidney procurement from DBD organ donation donors．