关键词:

Abstract:

Objective To explore the effect of serum HBsAg-positive expression on tumor recurrence of mouse in immunosuppressive status，thus to provide theoretical and experimental basis for the clinical practice of HBsAg-positive liver graft liver transplantation for patient with end-stage hepatocellular carcimona. Methods The experimental group（Group H）selects 30 hepatitis B virus（HBV）transgenic Balb/c mice，and the control group（Group D）selects 30 normal Balb/c mice. All of the mice were inoculated with H22 tumor cell under the spleenenvelope. After operation all of the mice were given CsA and methyl prednisolone sodium succinate to mimic the immunosuppressive status after liver transplantation. In postoperative 1，2 and 3 weeks，the mice were put todeath. Tumor recurrence rate and tumor load were compared between these two groups. Hematoxylin-Eosin（HE）staining and immunohistochemical detection of proliferating cell nuclear（PCNA），vascular endothelial growth factor （VEGF），inter-cellular adhesion molecule 1（ICAM-1）were performed. Results Tumor recurrence rates of the experimental group after 1，

2，3 weeks were 20%，50% and 80% respectively，with those of the control group were 20%，40% and 70% respectively. Tumor-bearing liver weight of the experimental group after 1，2，3 weeks was（1.04±0.05）g，（1.16±0.06）g and（1.29±0.06）g respectively，with those of the control group were（1.01±0.06）g， （1.13±0.05）g and（1.25±0.08）g respectively . The positive rates of PCNA in the liver tissue of the experimental group after 1，2，3 weeks were 22.20%±5.85%，44.20%±6.07% and 53.40%±7.92% respectively，with those ofthe control group were 22.80%±5.51%，43.60%±6.95% and 56.80%±9.14% respectively. The positive rates of VEGF of the experimental group after 1，2，3 weeks were 20.80%±5.47%，25.90%±5.51% and 37.60%±2.16%respectively，with those of the control group were 17.00%±4.14%，26.70%±6.40% and 37.30%±1.42%respectively. The positive rates of ICAM-1 of the experimental groups after 1，2，3 weeks were 15.40%±3.89%， 23.20%±3.61% and 31.30%±3.47% respectively，with those of the control group were 14.50%±3.41%，22.80%±2.97% and 29.40%±3.69% respectively. There were no statistical differences between these two groups（all P ＞ 0.05）. Conclusion By establishing the animal model of tumor recurrence in immunosuppressive status，we confirm that serum HBsAg-positive expression has no significant effect on tumor recurrence.