Objective This study aims to explore the practical experiences of a dedicated organ evaluation， maintenance，andprocurement team within the Organ Procurement Organization（OPO）in the context of disciplinary，systematic，and professional development，focusing on post-mortem organ donation，evaluation，maintenance，procurement，and repair. Methods This study analyzed the data of 780 donors from the First Affiliated Hospital of Xi'an Jiaotong University from September 1，2019 to August 31，2023，and compared the differences in basicinformation, surgical time, and organ cold ischemia of donors before and after the establishment of a dedicated team. Results The results indicated no significant differences in gender，age，blood type，or body mass index（BMI）between the two groups of donors. However，significant differences were observed in the primary diseases of donors，types of donation，duration of organ procurement surgery，and iatrogenic injuries. Conclusion The dedicated organ procurement team demonstrated superior performance in evaluating and maintaining organ function， effectively reducing both the duration of organ procurement surgery and organ cold ischemia time，while significantlydecreasing iatrogenic injuries during the organ procurement process. 

Objective To establish and evaluate a rapid response collaboration system for continuous renal replacement therapy（CRRT）in regional organ donors，assessing its clinical efficacy. Methods The case dataof this study were all from the Second Affiliated Hospital of Guangxi Medical University. This study retrospectively analyzed data from two groups ：the control group consisted of 29 organ donors who received conventional CRRT treatment between September 1，2020 and December 31，2021；the experimental group included 34 organ donors who were treated with the assistance of a CRRT rapid response medical team between January 1，2022，and December 31，2023. The differences in team response time，catheterization time，pipeline installation and pre-charging time，pre-machine preparation time，incidence of adverse events，incidence of complications，and equipment failure rates were compared between the two groups. Results Baseline data between the two groups were comparable （all P ＞ 0.05）. The experimental group demonstrated significantly shorter response times，catheterization times，pipeline installation and pre-charging times，and pre-machine preparation times compared to the control group，with statistically significant differences（all P ＜ 0.05）. No statistically significant differences were observed in the incidence of adverse events，complication rates，or equipment failure rates between the two groups（all P ＞ 0.05）. Conclusion The implementation of a CRRT rapid response team can substantially reduce treatment preparation time，enhance organ maintenance efficiency，and holds significant value for clinical. 

Objective To explore the correlation between deceased donor kidney hardness and hypothermic machine perfusion（HMP）parameters，providing a new quantitative indicator for assessing kidney quality and optimizing donor kidney selection criteria. Methods This study used a Shore hardness tester to quantitatively measure the hardness of 58 donated kidneys obtained from October 1, 2024 to November 30, 2024. Measurements were conducted both pre- and post-perfusion，and the flow rate and resistance index（RI）of HMP were systematicallyrecorded. Pearson correlation analysis was utilized to evaluate the relationship between kidney hardness and HMPparameters，providing a robust statistical framework for understanding their interdependencies. Results Preperfusion kidney hardness demonstrated a significant negative correlation with HMP flow rate（r ＝ -0.544，P ＜ 0.001） and a significant positive correlation with the resistance index（RI，r ＝ 0.745，P ＜ 0.001）. Similarly，post-perfusion kidney hardness showed a significant negative correlation with flow rate（r ＝ -0.477，P ＜ 0.001）and a significant positive correlation with RI（r ＝ 0.701，P ＜ 0.001）.Furthermore，the difference in kidney hardness before and after perfusion exhibited a significant positive correlation with flow rate（r ＝ 0.459，P ＜ 0.001）and a significant negative correlation with RI（r ＝ -0.524，P ＜ 0.001）. Conclusion There exists a significant correlation between donor kidney hardness and both flow rate and RI in HMP. Kidneys with higher hardness may be associated with microvascular injury or tissue fibrosis，leading to increase perfusion resistance and reduce flow rate during perfusion. The Shore durometer provides an objective and quantitative metric for assessing donor kidney quality. When combines with HMP parameters，it holds promise for optimizing donor kidney selection criteria and improving transplant success rates. 

Objective To analyze the impact of donor kidney Remuzzi score on short-term prognosis inadult single transplant recipients and identify independent risk factors for poor estimated glomerular filtration rate（eGFR，calculated using the CKD-EPI formula based on serum creatinine）recovery at 3 months post-transplant based on the Remuzzi scoring system. Methods Retrospective analysis of clinical data of 153 recipients at Southern Hospital of Southern Medical University from August 2021 to December 2023. Recipients were stratified by age into ＜ 50 years（n ＝ 98）group and ≥ 50 years（n ＝ 55）group，and each group was further divided into high Remuzzi score（＞ 3 points）group and low Remuzzi score（≤ 3 points）group. Dynamic eGFR（CKD-EPI formula）changes and post-transplant adverse events within 3 months，including post-transplant diabetes mellitus（PTDM）， urinary tract infection（UTI），viral infections〔cytomegalovirus（CMV），JC virus（JCV），BK polyomavirus（BKV）〕， delayed graft function（DGF），proteinuria，acute rejection，were compared between groups. Binary logistic regression was used to analyze risk factors for eGFR ＜ 60 ml/（min·1.73 m2 ）at 3 months post-transplant. Results In caseswith recipient age ＜ 50 years，the high Remuzzi score group exhibited significantly lower mean eGFR at 1 month （P ＝ 0.024）and 3 months（P ＜ 0.001）. Similar trends were observed in cases with recipient age ≥ 50 years （1 month ：P ＝ 0.003 ；3 months ：P ＝ 0.014）. Multivariate analysis identified Remuzzi score（OR ＝ 1.384，P ＝ 0.001）and recipient age（OR ＝ 1.052，P ＝ 0.006）as independent risk factors for poor eGFR recovery at 3 months post-transplant. Within the first 3 months post-transplant，recipients aged ＜ 50 years in the high Remuzzi score group exhibited a significantly higher incidence of JCV infection compared to the low Remuzzi score（21.1%vs. 3.8%，P ＝ 0.025）. However，no significant differences were observed between the groups in the incidence of PTDM，UTI，BKV infection，CMV infection，DGF，proteinuria，or acute rejection（all P ＞ 0.05）. In recipients aged ≥ 50 years，none of the adverse events demonstrated significant differences between the high- and low- score groups（all P ＞ 0.05）. Conclusion A donor kidney Remuzzi score ＞ 3 points independently predicts poor graft function recovery〔eGFR ＜ 60ml/（min·1.73 m2 ）〕at 3 months post-transplant in adult single transplant recipients. Combined with recipient age and donor kidney quality assessment，it facilitates postoperative risk stratification. Although the Remuzzi score lacks significant predictive value for short-term adverse events such as post-transplant diabetes mellitus and acute rejection，its association with increased JCV infection incidence in recipients ＜ 50 years warrants enhanced infection risk monitoring for high-score donor kidneys. 

Objective To investigate the impact of thoraco-abdominal organ procurement on thecontamination of abdominal organ preservation fluid and donor-derived infections related to organ preservation fluid in a single-center study. Methods A retrospective analysis was conducted on the clinical data of 98 organ donors who met the criteria and were admitted to the Affiliated Hospital of Qingdao University from January to December 2022. Those donors were divided into two groups based on organ procurement ：the thoraco-abdominal organ harvest group（35 cases）and the simply abdominal organ harvest group（63 cases）. The positive culture rates of trachealsuction sputum/bronchoalveolar lavage fluid and liver/kidney organ preservation fluid were analyzed. Additionally，the cultural consistency rate between organ preservation fluid and tracheal suction sputum/bronchoalveolar lavage fluid was evaluated by clinical physicians.Antibiotics and therapeutic outcomes in recipients with donor-derived infections related to organ preservation fluid were also assessed. Results A total of 102 organ donations were performed. 98cases met the inclusion criteria. The top five pathogens identified in donor respiratory tract cultures and liver/kidney organ preservation fluid cultures were as follows ：① Klebsiella pneumoniae，Pseudomonas aeruginosa，Acinetobacter baumannii，Staphylococcus aureus ；② Acinetobacter baumannii，Enterococcus faecium，Staphylococcus spp.，Yeast ； ③ Staphylococcus spp.，Enterococcus faecium，Yeast，Acinetobacter baumannii. Demographic analysis showed a significant difference only in age between the thoraco-abdominal and abdominal-only groups（40.60±11.82 vs. 53.30 ± 13.04）. In terms of culture results，a significant difference was observed only in the proportion of drug-resistant bacteria in liver organ preservation fluid in the thoraco-abdominal group（47.4% vs. 14.3%，χ2 ＝6.191，P＝0.013）. There were no significant differences in the positive rates of sputum culture（80% vs. 84.1%），liver and kidney organ preservation fluid（54.3% vs. 49.1%，75% vs. 58.9%），or the proportion of drug-resistant bacteria in kidney organ preservation fluid（54.2% vs. 30.3%）. The consistency rate defined by clinical physicians between organ preservation fluid and respiratory tract culture was significantly higher in the thoraco-abdominal group（26.5% vs. 6.5%）. Among the 13 donor-recipient pairs with consistent culture results，the early postoperative（within 1 month）donor-derived infection rate was 83.3% for both liver and kidney recipients，all of whom were successfully treated with prophylacticor salvage antibiotic therapy. Conclusion Although thoraco-abdominal organ procurement does not increase the contamination rate of organ preservation fluid，it does increase the likelihood of respiratory pathogens contaminating abdominal organs. Early post-transplantation antimicrobial strategies should be based on organ preservation fluid results while also considering respiratory tract culture results from heart and/or lung donors to enable timely prophylactic or salvage antibiotic use. 

Objective This study aims to observe the reparative effect of Necrostatin-1（NEC-1）ona rat model of moderate steatotic donor liver during NMP，and to preliminarily explore the mechanism of drug repair，providing experimental evidence for optimizing NMP strategies for steatotic donor livers. Methods This experiment included a total of 33 SD rats（male，8 ～ 10 weeks old），with 11 randomly selected for obtaining nonsteatotic liver as the liver source of animals，and the remaining 22 fed a high-fat diet for 2 months to induce steatotic liver confirmed by frozen section Oil Red O staining. The rats were divided into 3 groups ：Lean liver group（L group， n ＝ 6），steatotic liver group（S group，n ＝ 6），and steatotic liver group with addition of Necrostatin-1 to theperfusion solution（NS group，n ＝ 6），the 3 groups were preserved on a normothermic machine perfusion platform for 4 hours and analyzed by the infusion solution and liver tissue. Results During the perfusion process，the enzymatic elevation changes of NS group and S group were significantly higher than those of L group（P ＜ 0.05），NEC-1 had no significant effect on enzymatic changes（P ＞ 0.05），and the lactate concentration of NS group and S group was significantly higher than that of L group（P ＜ 0.05），but the lactate metabolism of NS group was effectively improved（P ＜ 0.05）. The triglyceride output of the NS group and the S group was significantly higherthan that of the L group（P ＜ 0.05），and the effect of NEC-1 on the secretion of triglycerides was not significant （P ＞ 0.05）. Conclusion Necrostatin-1 was able to improve the metabolic level of the rat model of steatosis donor liver in the experimental model of NMP ，and the repair effect of the drug on steatosis donor liver may be related to the downregulation of the necroptotic pathway. 

Objective To summarize the clinical characteristics of 17 cases of children with ureacirculation disorder who are scheduled for liver transplantation treatment，in order to improve the understandingof the disease. Methods The clinical data，laboratory examination data and imaging data of 17 children withurea circulation disorder who were treated in Beijing Friendship Hospital from January 2020 to January 2025 wereretrospectively analyzed. The current neurological development status of the children was evaluated by Gesell development scale，and the clinical characteristics of urea circulation disorder and related factors affecting prognosiswere discussed. Results The clinical data of 17 cases of children with urea circulation disorder were analyzed. One case（1/17）had onset in neonatal period，and the onset age was 15 days. 16 cases（16/17）had onset in nonneonatal period，with an average age of（20.9 ± 13.8）months ；There were 7 male patients（7/17）and 10 female patients（10/17）. The children with acute onset mainly showed mental retardation，vomiting and disturbance of consciousness. Most of the children with chronic onset showed liver function damage and underdevelopment. Among the 17 cases，all cases were accompanied by varying degrees of elevated liver enzymes（100%），15 cases were accompanied by varying degrees of elevated blood ammonia（88.2%），12 cases were accompanied by varying degrees of coagulation dysfunction（70.6%），and 3 cases were accompanied by varying degrees of elevated lactic acid （17.6%）. Nine cases（52.9%）showed hepatomegaly by abdominal imaging. Among the 17 cases，13 cases completed the cranial imaging examination，of which 5 cases showed specific changes（1 case clearly suggested the possibility of metabolic encephalopathy），and 8 cases showed no obvious abnormalities. Among the 17 cases，8 cases of Gesell developmental scale showed that the nervous system was stunted，and 5 cases showed that at least one developmental energy region was in a marginal state. The level of blood ammonia were related to the prognosis of nervous system development. Genotype can help to judge the prognosis of patients and guide the next birth. Conclusion urea cycle disorders often occur in infants and young children. Due to the nonspecific clinical manifestations，treatment is oftendelayed due to misdiagnosis as other diseases，and most survivors have varying degrees of nervous system damage. Hyperammonemia and elevated transaminase usually indicate the possibility of such diseases. It is necessary to be vigilant in clinic，and actively improve the hematuria metabolism screening and gene detection can help to make a clear diagnosis and judge the prognosis as soon as possible. Active control of blood ammonia level can effectively prevent further aggravation of central nervous system damage. Early liver transplantation can improve the prognosis of patients. 

 Objective To summarize the current status，research hotspots，and trends in heart transplant nursing in China，providing insights for clinical and research development. Methods A bibliometric analysis was conducted using CiteSpace based on relevant literature from CNKI，Wanfang，and VIP databases published between January 2000 and December 2023. Results A total of 309 articles were included. Although the publication volume was relatively low，the field has shown steady growth. Core journals have emerged，but stable research teams are lacking. Most studies were conducted by hospitals，with weak institutional collaboration. Key research areas includeperioperative care，psychological support，infection control，and rehabilitation，covering the full peri-transplant process. Conclusion Heart transplant nursing research in China is clinically driven，gradually shifting from basic care to refined management and long-term rehabilitation. Psychological care and infection prevention remainmajor focuses，while nursing for mechanical circulatory support is an emerging area. Future efforts should enhance evidence-based practice and multidisciplinary collaboration to promote high-quality development in the field.