Objective To evaluate the security and efficacy of liver transplantation for severe hepatitis. Methods Medical records of liver transplantations conducted between Jan 1st，2015 and Dec 31st， 2015 were collected and analyzed statistically. Results Before liver transplantation，the model for end-stage liver disease（MELD）score was higher in severe hepatitis group than that in non-severe hepatitis group 〔30.9（27.5，34.7）〕vs.〔17.0（15.0，18.9）〕. There was no significant difference in operation time，anhepatic phase， infection rate after transplantation，recurrence rate of hepatitis B or 1-year survival rate between groups. But the volume of blood transfusion during operation in severe hepatitis group was higher than that in non-severe hepatitis group（3 069.1±1 139.7 vs. 699.0±422.7）. Patients with severe hepatitis had longer postoperative hospitalization 〔37（33，44）vs. 30（28，33）〕. Conclusion Liver transplantation is safe for patients with severe hepatitis，and it is an effective treatment for severe hepatitis.

Objective To investigate the feasibility of interventional therapy for early portal vein thrombosis after liver transplantation. Methods Portal vein venography was performed in 2 patients with portal vein thrombosis to confirm the diagnosis of portal vein thrombosis. Balloon dilatation and stenting were conducted and blood flow was resumed after these treatments. Results All the 2 patients were treated effectively. No thrombosis occurred during the follow-up period. Conclusion Early portal vein thrombosis after liver transplantation can be treated safely and effectively through interventional therapy.

Objective To study the techniques in diagnosis and prevention of splenic artery steal syndrome （SASS）after liver transplantation（LT）. Methods A total of 159 patients who underwent LT in our hospital between Nov 2011 and Apr 2017 were studied，including five patients identified as SASS，and four patients receiving splenic artery ligation（SAL）as a way to prevent SASS. Patients with SASS were diagnosed by Doppler ultrasound and confirmed by subsequent digital subtraction angiography（DSA）. Four patients with SASSs were treated with splenic artery embolization（SAE），and 1 patients with SASS was treated with SAL. Platelet count ＜ 50×109 /L，the cealibre ratio of the splenic artery（SA）and common hepatic artery（CHA）＞ 1.5，portal vein velocity ＞ 50 cm/s were employed as the indication for SAL. Four patients at high risk for SASS received SAL to prevent SASS （pre-SAL）. Results None of the 4 patients who received pre-SAL developed SASS，and all of the 4 patients recovered smoothly. Three of five patients diagnosed as SASS after LT received SAE，and one patient with SASS received SAL. Onepatient developed hepatic artery thrombosisand received thrombolysis and SAE. All of the five patients with SASS recovered smoothly. Conclusion SASS is a serious complication after LT. Pre-SAL is an effective way to prevent SASS in patients at high risk. Timely diagnosis in early stage and salvage intervention of SAE or SAL are effective to prevent further progression and devastating consequences.

Objective To explore the method of repairing donor liver from donation after cardiac death （DCD）in vitro，using BMMSCs combined with normal mechanical perfusion（NMP）at room temperature to study the effect on the liver after cardiac death. Methods BMMSCs were cultured in vitro and the DCD rat model was established with a warm ischemia time of 45 min after cardiac death. Wistar rats were randomly divided into 3 groups: group A（cold storage）, group B（NMP alone）and group C（NMP + BMMSCs）. All the livers were preserved for 4 h （n ＝ 5）. Alanine aminotransferase（ALT）and aspartate transaminase（AST）in the circulating fluid were detected by a blood biochemical instrument, and the histological presentations of the livers were showed by a hematoxylineosin（HE）staining. Oxygen consumption was measured by a blood gas analyzer. Results The levels of ALT and AST in group C were significantly lower than those in group B（ALT ：t ＝ 177.98，P ＜ 0.05 ；AST ：t ＝ 284.38, P ＜ 0.05）or group A（ALT ：t ＝ 3 100.39，P ＜ 0.05 ；AST ：t ＝ 192.88，P ＜ 0.05）. The oxygen consumption of group C was significantly higher than that of group B after preservation for 1 h（4 h ：t ＝ 22.90，P ＜ 0.05）. Histological examination of liver showed that the histological presentation of group C was impoved in comparison with the other two groups. Conclusion The combining of BMMSCs and NMP has an obvious protective effect on the DCD liver, which was showed by ALT，AST，histological presentation and cell activity. It may serve as a new method to repair DCD liver graft.