Practical Journal of Organ Transplantation(Electronic Version) ›› 2016, Vol. 4 ›› Issue (1): 27-31.

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Vitexin:The protective effect of hepatic ischemia-reperfusion injury through the autophagy regulationin mice

  

  • Online:2016-01-20 Published:2021-06-25

牡荆素调节自噬对小鼠肝缺血 / 再灌注损伤的保护作用

于瑶 1 ,何金丹 1 ,王振 1 ,李世朋 1 ,喻文立 2 ,杜洪印 2

  

Abstract:

Objective To explore the effect of vitexin on hepatic ischemia-reperfusion injury(HIRI)by regulating autophagy in mice. Methods Eighteen male C57BL/6 mice weigh 20-25 g were selected anddivided into Sham group,Ischemia-Reperfusion(I/R)group and Vitexin group,respectively,with 6 mice in eachgroup. Vitexin group received intraperitoneal injection of vitexin 10 mg/kg 6 h preoperatively. Sham group and I/R group received equal amount of normal saline. HIRI model was constructed in I/R group and Vitexin group. Sham group received abdomen open and closure only. Serum alanine transaminase(ALT)and aspartate aminotransferase (AST)levels were detected in all groups,histopathological features were observed by hematoxylin-eosin(HE)staining,apoptosis was detected by Terminal-deoxynucleoitidyl Transferase Mediate Nick End Labeling (TUNEL)assay and expression of Beclin1,Bcl-2,LC3,caspase-3 proteins were detected by WesternBlot. Results Compared with I/R group,Vitexin group had a significantly lower levels of ALT and AST inserum〔ALT(U/L):201.57±77.12 vs. 444.31±92.61,AST(U/L):311.72±90.91 vs. 623.54±112.05,

all P 0.05〕,decreased injury of liver cells were shown in microscope and apoptotic cells marked by TUNEL assay,and the expressions of Beclin1,LC3,caspase-3 proteins weredecreased while

The expressionofBcl-2proteinincreased at the same time. Conclusion Vitexin canattenuate HIRI

inmice,whichmaybe related to theinhibitionof hepatocyteautophagy

摘要:

目的 探讨牡荆素(Vitexin)对小鼠肝缺血 / 再灌注损伤(HIRI)的作用并阐述可能的机制。方法 选取 18 只体重为 20 ~ 25 g 的健康雄性 C57BL/6 小鼠,随机分为假手术组(Sham 组)、缺血 / 再灌注组(I/R 组)和牡荆素组(Vitexin 组),每组 6 只小鼠。Vitexin 组于术前 6 h 腹腔注射 10 mg/kg 牡荆素。Sham 与 I/R 组腹腔注射等量生理盐水。I/R 组和 Vitexin 组建立小鼠肝脏 HIRI 模型,Sham 组仅进行开腹和关腹操作。检测各组小鼠血清中丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)水平,光镜下观察肝组织病理学改变,TUNEL 法检测肝细胞凋亡情况 ;通过蛋白质免疫印迹试验(Western Blot)检测自噬调控因子 Beclin1、抗凋亡相关基因 Bcl-2、微管相关蛋白轻链 3(LC3)与天冬氨酸特异性半胱氨酸蛋白酶 3(caspase-3)的表达水平。结果 与 I/R 组相比较,Vitexin 组小鼠血清 ALT 和 AST 水平明显降低〔ALT(U/L):201.57±77.12比 444.31±92.61,AST(U/L):311.72±90.91 比 623.54±112.05,均 P < 0.05〕,且在光镜下可见肝细胞损伤减轻 ;同时凋亡的肝细胞数量明显减少 ;肝组织中 Beclin1、LC3 和 caspase-3 蛋白的表达水平明显降低,而 Bcl-2 蛋白的表达水平明显增加。结论 牡荆素能够减轻小鼠 HIRI,其机制可能与牡荆素抑制再灌注时肝细胞自噬有关。