Abstract:

Objective Establish model of abdominal heterotopic heart transplantation in mice and summarize the experience to provide animal model support for further study of organ transplantation immunology. Methods Inbred BALB/c（n ＝ 30）and C57BL/6（n ＝ 30）mice were selected as donors，and inbred BALB/c（n ＝ 60）mice were used as recipients. The ascending aorta of the donor was anastomosed to the abdominal aorta of the recipient，and the pulmonary artery of the donor was anastomosed to the inferior vena cava of the recipient respectively to establish the heterotopic heart transplantation model. The survival time and the rejection of grafts were observed postoperatively. Results The successful rate of transplantation was 85%（51/60）. The donoroperation time was（7.0±1.0）min，and the recipient operation time was（60±10）min. The vascular anastomosis time was（25±3.0）min. After the transplantation，no immunosuppressive agent was used，and the survival time of the graft was（7.6±0.9）d. The graft on the fifth day，the seventh day showed typical rejection by histopathology. Conclusion Skilled microsurgical techniques and timely management of surgical complications are key to the successful establishment of abdominal heterotopic heart transplantation in mice. 

Key words:

Mice, Heart transplantation, Abdominal, Heterotopic

摘要：

目的 总结并改进小鼠腹部异位心脏移植模型构建的技术和经验，优化用于心脏移植免疫 排斥反应研究的动物模型。 方法 使用野生型 BALB/c 和 C57BL/6 小鼠各 30 只作为心脏移植手术供体， 近交系 BALB/c 小鼠 60 只作为心脏移植手术受体。分别吻合供体小鼠心脏和受体小鼠的主动脉和腹主动 脉，肺动脉和下腔静脉来构建腹部异位心脏移植模型。术后观察移植心脏的存活时间及移植物的排斥反应 情况。 结果 移植手术成功率为 85%。供体取心手术在（7.0±1.0） min 内完成，受体心脏移植手术约在 （60±10） min 内完成，其中吻合供受体血管用时约为（25±3.0） min。移植术后未用任何免疫抑制剂，同 种异品移植小鼠供心存活时间为（7.6±0.9） d。第 5 天、第 7 天取供心，病理组织学显示呈典型排斥反应。 结论 熟练的显微外科技术和及时处理手术并发症是成功建立小鼠腹腔心脏移植模型的关键。 

关键词:

小鼠 , 心脏移植 , 腹腔 , 异位