Practical Journal of Organ Transplantation(Electronic Version) ›› 2013, Vol. 1 ›› Issue (1): 44-46.

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The role of CD4+ T lymphocyte ATP assay in predicting infections after liver transplantation

WANG Kai,GAO Wei,ZHU Zhi-jun,SUN Li-ying,SUN Xiao-ye,QU Wei,SHEN Zhong-yang.
  

  1. TransplantDepartment of Tianjin First Center Hospital,Key Laboratory of Organ Transplantation of Tianjin,Key Laboratory of
    Critical Care Medicine of the Ministry of Health,Tianjin 300192,China
  • Online:2013-01-20 Published:2021-04-20

CD4+ T 淋巴细胞三磷酸腺苷检测在预测肝移植术后感染中的作用

王凯,高伟,朱志军,孙丽莹,孙晓叶,曲伟,沈中阳
  

  1. 天津市第一中心医院器官移植中心移植外科,卫生部危重病急救医学重点实验室,天津市器官移植重点实验室,天津 300192
  • 基金资助:

    国家高技术研究发展计划(863)项目(2012AA021008);

    国家临床重点专科建设项目;卫生部重点实验室项目;

    天津医科大学重点学科专项(2009xk34)

Abstract:

Objective To investigate the relationship between the CD4+T lymphocytes ATP level inperipheral blood and infections after liver transplantation(LT). Methods Total 142 samples were collected from101 patients' peripheral blood post-LT. CD4+T lymphocytes ATP value was analyzed by ImmuKnow immune cellfunction assay kits. According to the occurrence of infection and immune responses conditions,the patients post-LTwere divided into different groups and then the relationship between the level of CD4+T cells ATP and infection wasanalyzed. Results According to the clinical state,the patients were divided into infection group and non-infectiongroup. CD4+T lymphocytes ATP value was significantly different between the two groups(P = 0.001),which were236.0(41.0~512.0)μg/L and 371.5(9.0~1000.0)μg/L respectively. On the other hand,the patients post-LT weredivided into low immune response group,normal immune response group and high immune response according to the ATP levels of CD4+T lymphocytes in peripheral blood. The infection rates were 25.0%,13.6% and 0 in these groupsrespectively,which had significant statistic difference among three groups(P = 0.003). Conclusion The ATP value of CD4+T lymphocyte in peripheral blood was significantly correlated with occurrence of infection post-LT whichwould be helpful to conduct clinical therapy.

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摘要:

目的 探讨外周血 CD4+T 淋巴细胞内三磷酸腺苷(ATP)水平与肝移植术后感染之间的关系。方法 采集肝移植术后患者外周血样本 142 例次,应用 ImmuKnow 免疫细胞功能检测试剂盒检测 ATP。根据患者肝移植术后是否发生感染及不同的免疫应答状态分组,分析 CD4+T 淋巴细胞 ATP 水平与肝移植术后感染的关系。结果 根据患者临床状态将患者分为感染组和非感染组,其 ATP 值分别为 236.0(41.0~512.0)μg/L 和 371.5(9.0~1000.0)μg/L,两组比较差异有统计学意义(P = 0.001)。根据患者外周血CD4+T 淋巴细胞 ATP 水平将患者分为低免疫应答组、正常免疫应答组和高免疫应答组,其肝移植术后感染的发生率分别为 25.0%、13.6% 和 0,各组间移植术后感染的发生率比较差异有统计学意义(P = 0.003)。结论 外周血 CD4+T 淋巴细胞 ATP 值与肝移植术后感染间具有良好的相关性,有利于指导临床治疗。

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