Abstract:

Objective To summarize the technical difficulties and experience in the orthotopic liver transplant swine model without veno-venous bypass, to establish the highly reproducible and stabile swine model for liver transplant research. Methods Liver transplant swine model without veno-venous bypass were established bythe using of BAMA miniature pigs from Feb 2015 to Jun 2015. Hemodynamics，arterial blood gas analysis and changes in biochemical indicators were monitored during the operation. The time of anhepatic phase，operation time，the amount of blood loss，the amount of blood transfusion and postoperative survival rate were monitored. Results A totalnumber of 27 liver transplantation models were established in swines without veno-venous bypass. Mean cold ischemic time of the donor liver was（125.3±20.4）mins，the mean time of anhepatic phase and operation were（20.6±2.3）mins and（178.7±26.3）mins，respectively. Compared with pre-anhepatic phase，hemodynamics and metabolism changedrapidly during anhepatic phase，central venous pressure（CVP），mean arterial pressure（MAP），pH value and base excess decreased significantly〔CVP（cmH2O）：1.52±0.33 vs. 5.83±0.58，MAP（mmHg）：52.34±5.73 vs. 94.53±7.87，pH ：7.31±0.03 vs. 7.40±0.02，base excess（mmol/L）：-8.34±3.70 vs. 0.64±2.02，all P ＜ 0.05〕. Heart rate and serum potassium increased markedly.〔heart rate（times/min）：148.00±7.74 vs. 92.00±9.64，serum potassium（mmol/L）：4.82±0.52 vs. 4.13±0.37，all P ＜ 0.05〕. Hemodynamics and metabolism graduallyreturn to normal after portal vein and inferior vena cava were opened and blood flow restored. The 3-days and 7-days post-operation survival rates were 96.3% and 88.9% respectively. Conclusion Maintaining hemodynamics stable， decreasing the time of anhepatic phase and reducing the operative complication were the key points of establishing liver transplantation swine model without veno-venous bypass.