Objective To explore the role of tumor necrosis factor-α-induced Protein-8-like2（TIPE2）in T cells mediated acute cardiac rejection in mouse. Methods Hematoxylin-eosin（HE）staining and flow cytometry were used to identify the preface of immune cells in cardiac allograft rejection. Immunofluorescence staining was taken to determine the relationship between TIPE2 and splenocytes. Real-time PCR was used to detect the expression of TIPE2 and cytokine in cardiac allograft. Results Seven days after heart transplantation of mice, the histological features of myocardia of allograft were extensive necrosis and abundant infiltration of inflammatory cells. Flow cytometry showed upregulations of CD4+ and CD8+ T cells in cardiac allograft. CD4+ or CD8+ staining in splenocytes also showed the expression of TIPE2. Real-time PCR detected increased expressions of TIPE2 and cytokine in cardiac allograft. Conclusion TIPE2 might take part in T cells mediated acute cardiac rejection process.