实用器官移植电子杂志

实用器官移植电子杂志 ›› 2021, Vol. 9 ›› Issue (1): 50-55.DOI: 10.3969/j.issn.2095-5332.2021.01.012

• 论著 • 上一篇    下一篇

PPP2R3A 基因在乙肝相关性肝癌中的表达及临床意义

何佳佳 1，2，王培晓 3，矫宁 1，邱爽 1，朱雄伟 1，吴凤东 1，张庆 1，2   

  1. 1. 解放军总医院第三医学 中心器官移植科，北京 100039， 2. 安徽医科大学，安徽 合肥 230032， 3. 河南省儿 童医院消化内科，河南 郑州 450018
  • 出版日期:2021-01-20 发布日期:2021-06-25
  • 基金资助:

    国家自然科学基金（81372595）；

    国家重大科学研究计划（973）项目（2014CBA02001）

Clinical significance of PPP2R3A expression in hepatitis B virus related hepatocellular carcinoma

He Jiajia 1，2 ，Wang Peixiao 3 ，Jiao Ning 1 ，Qiu Shuang 1 ，Zhu Xiongwei 1 ，Wu Fengdong 1 ，Zhang Qing 1，2   

  1. 1.Department of Organ Transplant，The Third Medical Center of Chinese PLA General Hospital ，Beijing 100039，China， 2. Anhui Medical University，Hefei 230032，An Hui，China， 3. Institute of Gastroenterology，Henan Children's Hospital，Zhengzhou 450018， He Nan，China
  • Online:2021-01-20 Published:2021-06-25

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摘要：

目的 探讨蛋白磷酸酶 2A 调节亚基 B''α（protein phosphatase 2 regulatory subunit B″ alpha，PPP2R3A）在乙肝相关性肝癌中的表达及临床意义。方法 利用免疫组织化学方法检测 PPP2R3A 蛋白在70 例乙肝相关性肝癌和 40 例良性肝病组织中的表达，采用酶联免疫吸附试验（enzyme linked immunosorbentassay，ELISA）法检测 PPP2R3A 在肝癌患者外周血中的蛋白水平表达，运用统计学方法分析 PPP2R3A 蛋白水平的表达与乙肝相关性肝癌临床病理学指标的关系。结果 PPP2R3A 蛋白在肝癌组织中的表达主要定位于细胞质，其在肝癌组织中的表达明显高于癌周组织（P ＜ 0.001），并且 PPP2R3A 蛋白在肝癌组织中的表达明显高于良性肝病（P ＜ 0.001）。PPP2R3A 蛋白的表达强度与肝癌患者的 AFP 值（r ＝ 0.256，P ＝ 0.032）具有统计学差异。ELISA 结果也显示 PPP2R3A 蛋白在肝癌患者外周血中的表达量明显高于其在良性肝病中的表达（P ＝ 0.033），与免疫组织化学结果一致。结论 PPP2R3A 蛋白在乙肝相关性肝癌组织及血清中呈高表达，且与肝癌的临床病理特征有相关性，可能与肝癌的发生发展有关。

关键词: 乙肝相关性肝癌 , PPP2R3A 基因

Abstract:

Objective To investigate the expression and clinical significance of protein phosphatase2 regulatory subunit B'α（protein phosphatase 2 regulatory subunit B "alpha，PPP2R3A）in hepatitis B virus（HBV）related hepatocellular carcinoma （HCC）. Methods The expression of PPP2R3A protein in 70 casesof HBV related HCC and 40 cases of benign liver disease was detected by immunohistochemical method.The expression level of PPP2R3A in peripheral blood of patients with HCC was detected by enzyme linkedimmunosorbent assay （ELISA） method. The correlation between the expression of PPP2R3A protein and clinicopathological indexes of HBV related HCC was analyzed by statistical method. Results The expression of PPP2R3A protein in HCC tissues was mainly located in the cytoplasm， and the expressionof PPP2R3A protein in HCC tissues was significantly higher than that in peri-cancerous tissues（P ＜ 0.001），and the expression of PPP2R3A protein in HCC tissues was significantly higher than the liver tissueof benign liver diseases（P ＜ 0.001）. The expression intensity of PPP2R3A protein was statistically different fromthe AFP value of HCC patients（r ＝0.256，P ＝ 0.032）. The results of ELISA also showed that the expression of PPP2R3A protein in peripheral blood of patients with HCC was significantly higher than that in benign liver diseases（P ＝ 0.033），which was consistent with the results of immunohistochemistry. Conclusion PPP2R3A protein is highly expressed in the tissue and serum of HBV related HCC，and is related to the clinicopathological features of HCC，which may be related to the occurrence and development of HCC. 

Key words: HBV related HCC, PPP2R3A gene

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