Practical Journal of Organ Transplantation(Electronic Version) ›› 2020, Vol. 8 ›› Issue (6): 466-471.DOI: 10.3969/j.issn.2095-5332.2020.06.012

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The up-regulation of miR-423-3p in hepatitis B virus-related liver cancer liver transplantation and its effect on the prognosis of patients

Ke Ruisheng 1,2,Chen Chongfa3,Chen Yongtai2,Zhang Siyu1,Liu Zhaohui1,Cai Qiucheng4,Jiang Yi4,Lv Lizhi4.
  

  1. 1. Department of General surgery,the First Affiliated Hospital of Xiamen University,Xiamen 361001,Fujian,China ;
    2. Graduate School of Fujian Medical University,Fuzhou 350122,Fujian,China ;
    3. Xiamen University School ofMedicine,Xiamen 361001,Fujian,China ;
    4. Department of Hepatobiliary Surgery,900 Hospital of the Joint LogisticsTeam,Fuzhou 350025,Fujian,China
  • Online:2020-11-20 Published:2021-06-01

miR-423-3p 在乙型肝炎病毒相关肝癌肝移植组织中表达上调对患者预后的影响

柯瑞盛 1,2,陈重发 3 ,陈永泰 2 ,张思宇 1 ,刘昭晖 1 ,蔡秋程 4 ,江艺 4 ,吕立志 4
  

  1. 1. 厦门附属第一医院普外科,福建 厦门 361001 ; 2. 福建医科大学研究生院,福建 福州 350122 ; 3. 厦门大学医学院,福建 厦门 361001 ; 4. 中国人民解放军联勤保障部队第九〇〇医院(原南京军区福州总医院)肝胆外科,福建 福州 350025
  • 基金资助:

    福建省科技厅引导性项目(2019Y0068,2015Y0026);

    解放军后勤医学研究重点项目基金资助(CNJ15J002、14ZX22);

    福建医科大学科研启航基金项目(2017XQ2048)

Abstract:

Objective To detect the expression of miR-423-3p in hepatocellular carcinoma tissues andcells,to explore its role in the occurrence and development of liver cancer,and to explore whether it can be used as a potential molecular target for the detection and treatment of liver cancer in liver transplantation. Methods TheStarBase V 3.0 database was used to analyze the expression and prognostic value of miR-423 in liver cancer sample tissues,and quantitative real-time polymerase chain reaction(qRT-PCR)experimental method was applied to detect the expression of miR-423-3p in 106 cases of liver cancer liver transplantation tissues and in 30 normaladjacent liver tissues. The relationship between the expression of miR-423-3p and the pathological characteristicsand prognosis of liver cancer patients undergoing liver transplantation was analyzed based on the clinical data of thepatients. Results Compared with adjacent non-tumor tissues,qRT-PCR detected that miR-423-3p expression was increased in liver cancer tissues. The analysis of clinicopathological characteristics of HCC patients showed that miR-423-3p expression was closely related to tumor-free envelope,larger tumor diameter,lower differentiation and higher TNM stage(P < 0.05). Further COX multivariate retrospective analysis revealed that miR-423-3p expression,degree of differentiation and TNM stage were independent prognostic risk factors for patients with poor prognosis(P < 0.05). Using Kaplan-Meier analysis,the cumulative survival rate of miR-423-3p overexpressing liver cancer patients was significantly lower than that of patients with low expression(P < 0.01). Conclusion Overexpression of miR-423-3p may be involved in the occurrence and development of HBV-related HCC,and it can be used as a prognostic marker and clinical target for liver cancer patients with liver transplantation.

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摘要:

目的 检测 miR-423-3p 在肝癌样本组织中的表达情况,探讨其可否作为乙型肝炎病毒(hepatitis B virus,HBV)肝细胞癌(hepatocellular carcinoma,HCC)肝移植患者诊疗的潜在分子靶标。方法 StarBase v3.0 数据库分析 miR-423 在肝癌样本组织中表达及预后价值,采用实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR)实验方法检测 106 例肝癌肝移植组织和30 例正常癌旁肝组织中 miR-423-3p 表达,结合患者的临床资料分析 miR-423-3p 表达与肝癌行肝移植患者病理特征和预后生存的关系。结果 与邻近正常组织相比,qRT-PCR 实验检测发现在肝癌组织中 miR-423-3p 表达增高。临床病理学特征分析结果表明 miR-423-3p 表达与无肿瘤包膜、较大肿瘤直径、较低分化程度和较高 TNM 分期密切相关(P < 0.05)。进一步 COX 多因素回顾分析发现 miR-423-3p 表达,分化程度,TNM 分期等是患者预后不良的独立预后危险因素(P < 0.05)。采用 Kaplan-Meier 方法和秩和检验进行统计分析,miR-423-3p 过表达肝癌肝移植患者累积生存率显著低于低表达患者(P < 0.01)。 结论 miR-423-3p 可能参与肝癌的发生和发展过程,可以作为肝癌肝移植患者疾病预后标志物和临床治疗靶标。

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