Abstract:

Objective To detect the expression of miR-423-3p in hepatocellular carcinoma tissues andcells，to explore its role in the occurrence and development of liver cancer，and to explore whether it can be used as a potential molecular target for the detection and treatment of liver cancer in liver transplantation. Methods TheStarBase V 3.0 database was used to analyze the expression and prognostic value of miR-423 in liver cancer sample tissues，and quantitative real-time polymerase chain reaction（qRT-PCR）experimental method was applied to detect the expression of miR-423-3p in 106 cases of liver cancer liver transplantation tissues and in 30 normaladjacent liver tissues. The relationship between the expression of miR-423-3p and the pathological characteristicsand prognosis of liver cancer patients undergoing liver transplantation was analyzed based on the clinical data of thepatients. Results Compared with adjacent non-tumor tissues，qRT-PCR detected that miR-423-3p expression was increased in liver cancer tissues. The analysis of clinicopathological characteristics of HCC patients showed that miR-423-3p expression was closely related to tumor-free envelope，larger tumor diameter，lower differentiation and higher TNM stage（P ＜ 0.05）. Further COX multivariate retrospective analysis revealed that miR-423-3p expression，degree of differentiation and TNM stage were independent prognostic risk factors for patients with poor prognosis（P ＜ 0.05）. Using Kaplan-Meier analysis，the cumulative survival rate of miR-423-3p overexpressing liver cancer patients was significantly lower than that of patients with low expression（P ＜ 0.01）. Conclusion Overexpression of miR-423-3p may be involved in the occurrence and development of HBV-related HCC，and it can be used as a prognostic marker and clinical target for liver cancer patients with liver transplantation.

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摘要：

目的 检测 miR-423-3p 在肝癌样本组织中的表达情况，探讨其可否作为乙型肝炎病毒（hepatitis B virus，HBV）肝细胞癌（hepatocellular carcinoma，HCC）肝移植患者诊疗的潜在分子靶标。方法 StarBase v3.0 数据库分析 miR-423 在肝癌样本组织中表达及预后价值，采用实时荧光定量聚合酶链反应（quantitative real-time polymerase chain reaction，qRT-PCR）实验方法检测 106 例肝癌肝移植组织和30 例正常癌旁肝组织中 miR-423-3p 表达，结合患者的临床资料分析 miR-423-3p 表达与肝癌行肝移植患者病理特征和预后生存的关系。结果 与邻近正常组织相比，qRT-PCR 实验检测发现在肝癌组织中 miR-423-3p 表达增高。临床病理学特征分析结果表明 miR-423-3p 表达与无肿瘤包膜、较大肿瘤直径、较低分化程度和较高 TNM 分期密切相关（P ＜ 0.05）。进一步 COX 多因素回顾分析发现 miR-423-3p 表达，分化程度，TNM 分期等是患者预后不良的独立预后危险因素（P ＜ 0.05）。采用 Kaplan-Meier 方法和秩和检验进行统计分析，miR-423-3p 过表达肝癌肝移植患者累积生存率显著低于低表达患者（P ＜ 0.01）。 结论 miR-423-3p 可能参与肝癌的发生和发展过程，可以作为肝癌肝移植患者疾病预后标志物和临床治疗靶标。

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