Abstract:

Objective To investigate the promoting effect of human umbilical cord mesenchymal stem cells（hUC-MSCs）on long-term cultured human islets. MethodsThe hUC-MSCs were divided into three groups according to different cell concentrations ：group 104 ，group 105 and group106 . Each group was inoculated into 12-well plates in three parallel wells. After culturing for 24 h，150 IEQ human islets cultured for 17 d were added to each well and the group cultured without hUC-MSCs was a control. After culturing at 37℃ and 5% CO2 for 24 h，the islet shape，activity and static glucose-stimulated insulinreleaseindex（GSI）werecomparedindifferent groups.Results After culture，the shapes islets ineach group were compac，and there were somedeadcellsfloating in the culture medium of group 106 . Theactivity ofisletsingroup105 was significantly higher than that in the control group（P ＜ 0.01），and the activity of islets in group 106 was relatively poor（P ＜ 0.01）. The glucose stimulation GSI of pancreatic islets in group 105 was significantly higher than that of the control group（3.63±0.16 vs. 2.52±0.10，P ＜ 0.01）. There was no significant difference in the GSI of human pancreatic islets between the other two groups and the control group. ConclusionhUC-MSCs canimprove the in vitro activity and function of human pancreaticislet cell mass after long-term culture，but it is related to the concentration ofMSCs. 

Key words:

Human umbilical cord mesenchymal stem cells , Human pancreatic islets , Long-term culture, Function

摘要：

目的 探讨人脐带间充质干细胞（human umbilical cord mesenchymal stem cells，hUC-MSCs）对长期培养的人胰岛功能的改善作用。 方法 将 hUC-MSCs 根据不同浓度分成 3 组：104 组、105 组、106 组，每组平行三孔接种于 12 孔板中，经培养 24 h 贴壁后，各孔分别加入 150 IEQ 培养了 17 d 的人胰岛，以无hUC-MSCs 的胰岛培养为对照组。经 37℃，5%CO2 培养 24 h 后，比较不同组胰岛外形、活性和静态葡萄糖刺激胰岛素释放指数（insulin release index，GSI）。 结果 培养后，各组胰岛形态紧实，106 组培养液有较多细胞成膜片漂浮，105 组胰岛活性显著高于对照组胰岛（P ＜ 0.01），106 组胰岛活性相对较差（P ＜ 0.01）。105 组胰岛的葡萄糖刺激 GSI 与对照组具有显著性差异（3.63±0.16 比 2.52±0.10，P ＜ 0.01），其余两组人胰岛GSI与对照组无显著性差异。 结论 hUC-MSCs可以改善长期培养后人胰岛细胞团的体外活性和功能，但与 MSCs 细胞浓度有关。

关键词:

人脐带间充质干细胞 , 人胰岛 , 长期培养 , 功能