Abstract:

Objective To evaluate the significance of detecting cytomegalovirus antigenemia in thediagnosis of cytomegalovirus infection in renal transplant patients. Methods From September 2021 to December2021，a total number of 110 blood samples from 90 renal transplant patients in The First Hospital of Kunming were collected.The CMV pp65 antigenemia test was compared with the results of IgG antibody and CMV DNA. The correlation betweenthe CMV pp65 antigenemia results and creatinine，tacrolimus（tacrolimus，Tac/FK506）concentration，lymphocyte subsetswas evaluated. Results The consistency between CMV pp65 antigenemia results and CMV DNA results was 65.5%，the difference was statistically significant（P ＜ 0.05）. The consistency between the CMV pp65 antigenemia test resultsand the CMV IgG antibody test results was 57.3%，the difference was not statistically significant（P ＞ 0.05）. There wasno significant difference between CMV pp65 antigenemia results and creatinine as well as FK506 drug concentrations（P ＞ 0.05）. In the comparison between CMV pp65 antigenemia results and lymphocyte subsets，there was only astatistically significant difference between CMV pp65 and NK cell absolute value（P ＜ 0.05）. Conclusion Theconsistency between CMV pp65 antigen and CMV IgG antibody was 57.3%. The consistency between CMV pp65 antigenand CMV DNA was 65.5%. The combined detection of CMV pp65 antigen and CMV DNA has certain significance inimproving the accuracy of clinical diagnosis of CMV infection. There was no statistically significant difference in the serumcreatinine level and the whole blood FK506 drug concentration between the CMV pp65 antigen negative group and thepositive group. The absolute value difference of NK cells was statistically significant. The probability of CMV infection inrenal transplant patients with high absolute value of NK cells was low.

Key words:

Cytomegalovirus, pp65 antigenemia, Renal transplantation

摘要：

目的 评估巨细胞病毒抗原血症检测方法对诊断肾移植患者巨细胞病毒感染的意义。方法 收集 2021 年 9 月至 2021 年 12 月昆明市第一人民医院就诊 90 例肾移植患者的 110 例血样，对其进行巨细胞病毒（cytomegalovirus，CMV）pp65 抗原血症检测与 IgG 抗体、CMV DNA结果比较，评估 CMV pp65 抗原血症结果与肌酐、他克莫司（tacrolimus，Tac/FK506）药物浓度、淋巴细胞亚群的相关性。结果 CMV pp65 抗原血症结果与 CMV DNA 结果一致性为 65.5%，差异具有统计学意义（P ＜ 0.05）。CMV pp65 抗原血症检测结果与CMV IgG 抗体检测结果一致性为 57.3%，差异不具有统计学意义（P ＞ 0.05）。CMV pp65 抗原血症结果与肌酐、FK506 药物浓度比较，差异无统计学意义（P ＞ 0.05）。CMV pp65 抗原血症结果与淋巴细胞亚群比较中，仅与NK 细胞绝对值差异有统计学意义（P ＜ 0.05）。结论 CMV pp65 抗原与 CMV IgG 抗体结果一致性为 57.3%。CMV pp65 抗原与 CMV DNA 结果一致性为 65.5%，两种方法联合检测，对提高 CMV 感染临床诊断准确率具有一定意义。在CMV pp65抗原阴性组与阳性组中，血清肌酐水平、全血FK506药物浓度两组间差异无统计学意义，NK 细胞绝对值差异具有统计学意义，NK 细胞绝对值偏高的肾移植患者，发生 CMV 感染的概率偏低。

关键词:

巨细胞病毒 , pp65 抗原血症 , 肾移植