Abstract:

Objective To analyze the results of the determination of Tacrolimus（Tac）concentration in wholeblood by chemiluminescent microparticle immunoassay and latex enhanced immunosuppression. Methods The whole blood concentration of tacrolimus in 96 renal transplant recipients was parallelly measured by chemiluminescentmicroparticle immunoassay and latex enhanced immunosuppression, the results of different tests were compared and the correlation and regression were analyzed. Results The concentration of tacrolimus measured bychemiluminescent microparticle immunoassay（5.78 ± 2.18）μg/L was lower than that measured by latex enhanced immunosuppression（6.51±2.32）μg/L, and the difference was statistically significant（P ＜ 0.001）. There was a significant positive correlation between the two methods（r ＝ 0.9676，P ＜ 0.0001）, besides, the results of the twomethods were in accordance with the linear regression equation Y ＝ 0.551+1.032X（X was the chemical radio-particle immunoassay, Y was the latex enhanced immunosuppression）. Conclusion There was a good correlationbetween the two methods, but due to the significant  difference, comparison between each other cannot be made. The two methods can be calculated by linear regression equation. It is suggested that the same method should be selected for the same patient to monitor the whole blood concentration of tacrolimus in the long run, so as to provide accurate and reliable basis for clinical individual drug application.

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摘要：

目的 分析化学发光微粒子免疫法和胶乳增强免疫抑制法检测他克莫司（Tacrolimus，Tac）全血药物浓度的结果。方法 用化学发光微粒子免疫法和胶乳增强免疫抑制法平行检测 96例肾移植受者他克莫司全血药物浓度，对比检测结果差异性并进行相关和回归分析。结果 采用化学发光微粒子免疫法测得的 Tac 血药浓度（5.78±2.18）μg/L 低于采用胶乳增强免疫抑制法测得的 Tac 血药浓度（6.51±2.32）μg/L，且差异具有统计学意义（P ＜ 0.001）；两种方法检测 Tac血药浓度结果呈显著正相关（r＝ 0.9676，P ＜ 0.0001）；两种方法检测 Tac 血药浓度 结果符合一元线性回归方程 Y ＝ 0.551+1.032X （其中 X 为化学放光微粒子免疫法，Y 为胶乳增强免疫抑制法）。结论 两种方法检测 Tac 血药浓度具有很好的相关性，但差异显著，不可相互比较，二者可通过一元线性回归方程来互相推算，建议同一患者长期选用同一种方法监测 Tac 血药浓度，以便为临床提供准确可靠的个体化用药依据。

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