Objective To detect the functional significance of surface inhibitory receptor leukocyteassociated immunoglobulin like receptor-1（LAIR-1）of NK cells in xenogeneic immune rejection. Methods The infiltration of immune cells was detected by the immunohistochemical staining in the donor liver tissue after xenotransplantation. The soluble LAIR-1（sLAIR-1）in the recipient plasma and membrane LAIR-1（mLAIR-1）in recipient immune cells were detected by the enzyme linked immunosorbent assay（ELISA）and RNA sequencing， respectively. Results In tissue level, the donor liver was infiltrated of lymphocytes. In molecular level, the sLAIR-1 and mLAIR-1 were in equilibrium. Conclusion The mLAIR-1of NK cell and sLAIR-1 in plasma were in balance and related to xenogeneic immune rejection. Taken all together these data suggest that the LAIR-1 represents an important target in inducing immunological tolerance.

目的 探讨 NK 细胞表面抑制性受体白细胞相关免疫球蛋白样受体 -1（eukocyte-associated immunoglobulin like receptor-1，LAIR-1）在异种移植免疫排斥反应中的作用。方法 采用免疫组织化学 染色检测异种移植术后供肝组织的免疫细胞浸润情况，酶联免疫吸附试验（enzyme linked immunosorbent assay，ELISA）检测受体血浆中可溶性 LAIR-1（sLAIR-1）的表达，转录组测序检测受体免疫细胞表面 膜型 LAIR-1（mLAIR-1）的表达。结果 异种移植术后供肝组织受到淋巴细胞浸润，受体淋巴细胞表面 mLAIR-1 和 sLAIR-1 呈动态平衡状态。结论 受体免疫细胞表面 mLAIR-1 和血浆中 sLAIR-1 的表达水平 处于动态平衡的状态可能与受体发生免疫排斥反应相关，LAIR-1 有望成为移植术后诱导免疫耐受的分子。