Objective To observe the role of the donor specific antibody（DSA） and human leukocyte antigen（HLA） antibody in pediatric liver transplantation. Methods The clinical data of liver transplantation cases in some children (aged below 18 years) that were performed between Sep 1 2016 and Dec 31 2016 in Tianjin First Central Hospital，were analyzed retrospectively. HLA antibodies were detected by Luminex before liver transplantation, 1 week after surgery and 3 months after surgery. HLA typing was detected in HLA antibody positive specimens. The incidences of rejection, cytomegalovirus （CMV） infection, EB virus infection, vascular complications and biliary complications after liver transplantation were observed. Results A total number of 11 cases were included. DSA was found positive in 1 case before liver transplantation, and in 1 case after liver transplantation. HLA antibodies of 2 cases were positive (non-DSA) before liver transplantation and negative after liver transplantation. The tests for HLA antibody of 1 case were positive before liver transplantation，one week and 3 months after operation. The HLA antibody in 1 case was positive 1 week after liver transplantation. HLA antibody in 5 cases was negative before and after liver transplantation. The average follow-up time of 11 patients was 15.3±1.9 months. All patientssurvived and no rejection was observed after liver transplantation. One case presented hepatic artery thrombosis on the third day and hepatobiliary anastomostic bile leakage occurred on the sixth day after liver transplantation. One case of anastomotic stenosis was found on the third day after liver transplantation. There were two cases of Cytomegalovirus infection and one case of EB virus infection after surgery. Conclusion Detection of DSA and HLA antibody in the early period of pediatric liver transplantation complications.has a certain significant

目的 观察小儿肝移植术后早期供体特异性抗体（DSA）及人类白细胞抗原（HLA）抗体对 肝移植术后并发症的影响。方法 回顾性分析天津市第一中心医院 2016 年 9 月 1 日 - 2016 年 12 月 31 日 部分小儿（年龄 18 岁以下）肝移植病例的临床资料。分别在肝移植术前、术后 1 周、术后 3 个月采用 Luminex 法检测 HLA 抗体，对于 HLA 抗体阳性标本检测 HLA 分型。观察患儿肝移植术后的排斥反应、巨 细胞病毒（CMV）感染、EB 病毒感染、血管并发症及胆管并发症等发生情况。结果 共纳入 11 例，肝 移植术前有 DSA 阳性 1 例，肝移植术后 DSA 阳性 1 例 ；肝移植术前 HLA 抗体阳性（非 DSA），肝移植术 后阴性 2 例 ；肝移植术前、术后 1 周及术后 3 个月 HLA 抗体均阳性 1 例 ；肝移植术后 1 周 HLA 抗体阳性 1 例 ；患者肝移植术前、术后 HLA 抗体持续阴性 5 例。11 例平均随访时间为（15.3±1.9）个月，均存活， 肝移植术后均未出现排斥反应。1 例在肝移植术后第 3 天出现肝动脉血栓，并在肝移植术后第 6 天出现胆 管吻合口胆瘘 ；1 例在肝移植术后第 3 天门静脉吻合口狭窄 ；2 例肝移植术后 CMV 感染，1 例 EB 病毒感染。 结论 小儿肝移植术后早期监测 HLA 抗体及 DSA 具有一定临床意义。