Abstract:

Objective To investigatethe procedure improvement of building a stable and operational orthotopic renal transplantation model in rats. Methods The left kidney was removed from the donor rat after perfusing it with ice-cold heparinized salinethrough the abdominal aorta. The donor kidney was transplantedorthotopically into a bilaterally nephrectomized recipient by end-to-end anastomosis of the left renal vessels andthe left ureter using microsurgical technique. Results According to the improved procedure，a stable and easily operational renal transplantation model was set up with a success rate above 80%. 10 pairs of rats have received renal transplantation surgeries till now. The mean total surgery time was（211.00±13.24）minutes，with donor nephrectomy of（87.00±5.34）minutes and renal transplantation of（120.00±7.90）minutes. During the transplantation surgery，the time of nephrectomies was（27.00±1.12）minutes，with artery anastomosis of（35.00±1.98）minutes，vein anastomosis of（36.00±3.26）minutes，and ureteroureterostomy of（22.00±1.54）minutes. The mean warm ischemia time was 30 seconds，and the mean cold ischemia time was 100 minutes. 3-day survival rate was 80% with good graft function. 2 recipients died of anastomotic bleeding，thrombosis，urine leakage，and so on. Conclusion With the procedure improvement，it was very helpful to set up a stable and operational rat renal transplantation model forimmunological researches.coping styles of liver transplant recipients were 19.38±3.98，15.01±3.17 and 8.01±2.67，respectively. The score of avoidance was significantly higher than that of the Feifel norm（P ＝ 0.008），whereas the score of confrontation and acceptance-resignation were significantly lower than that of the Feifel norm（P ＝ 0.046 and P ＝ 0.001 respectively）. The physical component summary（PCS）and mental component summary（MCS）scores of the SF-36 were 50.96±6.80 and 50.10±9.22，respectively. The recipients had lower scores of physical function（PF），domainsof body pain（BP）and general health（GH）compared to the norm（P ＜ 0.05）. However，there were no statistical differences between the recipients and norm of the scores of role-physical（RP），regarding vitality（VT），social functioning（SF），role-emotional（RE）（P ＞ 0.05），while the mental health（MH）

score was significantly higher than that of the norm（P ＜ 0.01）. The QOL scores in liver transplant recipients were negatively correlated with acceptance-resignation scores（r ＝ -0.310 to -0.542，P ＜ 0.05 or P ＜ 0.01），but not correlated with confrontation or avoidance scores. Conclusion Liver transplant recipients have significantly better MCS domain scores than PCS domain scores. Acceptance-resignation coping mode has negative impact on the QOL of liver transplantation recipients.

摘要：

目的 探讨建立一种稳定、操作性好的大鼠原位肾移植模型的外科技术及其改进方法。方法 用 4℃肝素生理盐水原位灌注供肾，手术显微镜下行供肾动脉、静脉、输尿管与受鼠同名血管及输尿管端端吻合。受鼠双侧肾脏一起切除，建立大鼠左侧原位肾移植模型。结果 经过技术改进，建立了稳定、大鼠单侧原位肾移植模型，模型成功率可达 80% 以上。对已完成的 10 对大鼠移植时间进行统计，完成 1 对大鼠肾移植需要（211.00±13.24）分钟，其中供肾切取（87.00±5.34） 分钟，供肾移植（120.00±7.90）分钟；供肾移植中原肾脏切除（27.00±1.12）分钟，动脉吻合（35.00±1.98）分钟，静脉吻合（36.00±3.26）分钟，输尿管吻合（22.00±1.54）分钟。供肾热缺血时间为 30 秒，肾脏冷缺血时间为 100 分钟。10 对原位肾移植大鼠中 8 对大鼠存活时间大于 3 天，移植肾形态、功能良好，其余2 对大鼠死亡原因包括吻合口出血、血栓形成、尿漏等。结论 本文所述方法和改进技巧有利于建立一种稳定的、适用于器官移植免疫学研究的大鼠原位肾移植模型。

关键词: