Abstract:

Objective To establish an ex situ machine perfusion（MP）system for long-term liverperfusion using normothermic machine perfusion（NMP）technology. Methods A porcine liver was connectedto the Life-X100 perfusion system for normothermic machine perfusion. Perfusion parameters and perfusate blood-gas analysis results were continuously monitored during the perfusion process to assess the ex situ liverfunction. Results The porcine liver was successfully perfused ex situ for 138 hours，with stable perfusionparameters and well-maintained liver function. Conclusion We have preliminarily established an ex situ longterm liver perfusion system. This system can be used for the ex situ maintenance of human livers in the future and can serve as a basis for various exploratory studies to advance medical development in our country. 

Key words:

Long-term normothermic machine perfusion , Preservation of liver in vitro , Organ function maintenance, Organ medicine

摘要：

目的 使用常温机械灌注（normothermic machine perfusion，NMP）建立离体肝脏长期灌注系统（ex situ MP for long-term liver perfusion）。 方法 将 1 例子猪全肝连接至 Life-X100 灌注系统进行常温机械灌注，持续监测灌注过程中灌注参数及灌注液血气分析结果，评估离体肝脏的功能。 结果 成功离体灌注猪肝 138 h，各项灌注参数稳定，离体肝脏功能维持良好。 结论 我们初步建立了离体肝脏长期灌注系统，在未来可用于人类肝脏的离体养护，并在此基础上进行多项探索性研究，促进我国医学发展。

关键词:

长期常温机械灌注 , 离体肝脏保存 , 器官功能维护 , 器官医学