Practical Journal of Organ Transplantation(Electronic Version) ›› 2024, Vol. 12 ›› Issue (3): 247-249.DOI: 10.3969/j.issn.2095-5332.2024.03.011

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Experimental observation on the inhibitory effect of metronomic capecitabine on acute rejection in rat liver transplantation

Li Shanni1 Chen Tao2 Wang Zhenglu3 Zheng Hong1 .    

  1. 1. Department of Transplantation Oncology, Tianjin First Central Hospital, Tianjin 300192,China;

    2. School of Medicine, Nankai University, Tianjin 300071,China;

    3. Tianjin First Central Hospital Biological Sample Library, Tianjin 300192,China.

  • Online:2024-05-20 Published:2024-05-20

节拍剂量卡培他滨抑制大鼠肝移植急性排斥反应的实验观察

李姗霓 1 ,陈涛 2 ,王政禄 3 ,郑虹   

  1. 1.天津市第一中心医院移植肿瘤科,天津 300192;

    2. 南开大学医学院,天津 300071 ;

    3. 天津市第一中心医院生物样本库,天津 300192

  • 基金资助:

    天津市卫生健康委员会科技项目(ZC20089)

Abstract:

ObjectiveTo observe the effects of metronomic capecitabine (CAP) on immune cells and rejection in acute liver transplant rejection in rats, and to explore its immunosuppressive effect. Methods A rat liver transplantation acute rejection model was established and randomly divided into a control group (CON group), a metronomic capecitabine group (CAP group), and a metronomic capecitabine+tacrolimus(Tac) group (CAP+Tac group). Venous blood was collected on the 1st,3rd, and 7th day after liver transplantation, and enzyme-linked immunosorbent assay (ELISA) was used to detect IL-2 and IFN-γ concentrations, the trends of changes and correlations of IL-2 and IFN-γ between groups. Results Compare to the CON group,the concentration of serum IL-2 and IFN- γ gradually decreased in the CAP and CAP+Tac groups on the 1st,3rd,and 7th day after transplantation, and there was a statistically significant difference between the these two groups and the CON group (P < 0.05). ConclusionThe metronomic capecitabine can reduce peripheral blood IL-2 and IFN-γ during acute rejection of liver transplantation in rats, it has potential immunosuppressive effects. 

Key words:

Capecitabine; Liver transplantation; Acute rejection reaction;Immunosuppression ,

摘要:

目的 观察节拍剂量卡培他滨(capecitabine,CAP)对大鼠肝移植急性排斥反应免疫细胞及排斥反应的影响,探讨其免疫抑制作用。方法 建立大鼠肝移植急性排斥反应模型,分为对照组(CON 组)、节拍剂量 CAP 组(CAP 组),节拍剂量 CAP+ 他克莫司(tacrolimus,Tac)组(CAP+Tac 组),分别于肝移植术后第 1、3、7 天采集静脉血,采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)方法检测 IL-2和 IFN-γ 含量,分析组间白细胞介素 -2(interleukin-2,IL-2)、γ- 干扰素(γ-interferon,IFN-γ)变化趋势及相关性。结果 肝移植术后第 1、3、7 天 ,CAP 组和 CAP+Tac 组血清 IL-2 和 IFN-γ 含量较对照组逐渐降低,较 CON 组有统计学差异(P < 0.05)。结论 节拍剂量 CAP 可降低大鼠肝移植急性排斥反应期外周血IL-2 和 IFN-γ 的分泌,具有免疫抑制作用。 

关键词:

卡培他滨 , 肝移植 , 急性排斥反应 , 免疫抑制