Objective To investigate the correlation between chromodomain helicase DNA-binding protein 8（CHD8）and the clinical pathological features as well as postoperative recurrence of hepatitis B virus associated hepatocellular carcinoma（HCC）. Methods The clinical pathological data and long-term follow-up after surgery data of 251 patients with hepatitis B related HCC who underwent liver transplantation from January 2007 to June 2012 in the Third Medical Center of Chinese PLA General Hospital were analyzed retrospectively. The expression of CHD8 protein in liver specimens was detected by immunohistochemistry. The correlation between CHD8 protein and clinical pathological indexes，such as HBeAg，postoperative recurrence and metastasis were analyzed. Results The expression of CHD8 protein in tumor tissue was lower than that in adjacent tissues（P ＝ 0.00），it was significantly correlated with microsatellite（P ＝ 0.00），vascular invasion（P ＝ 0.00），metastasis of other parts（P ＝ 0.00）. The expression of CHD8 protein was also associated with HBeAg（P ＝ 0.04），recurrence（P ＝ 0.01）and lung metastasis （P ＝ 0.04）. Conclusion The expression of CHD8 protein is associated with metastasis and recurrence after liver transplantation for hepatitis B virus associated HCC，and it maybe a potential marker for tumor recurrence and metastasis.

目的 探讨染色体结构域解旋酶 DNA 结合蛋白 8（chromodomain helicase DNA-binding protein 8， CHD8）与乙肝相关性肝癌（hepatocellular carcinoma，HCC）的临床病理学特征及术后肿瘤复发的相关性。 方法 回顾性分析 2007 年 1 月—2012 年 6 月期间，解放军总医院第三医学中心收治的 251 例肝移植术的乙 肝相关性 HCC 患者的临床病理资料及长期随访资料，采用免疫组织化学法检测肝癌标本中的 CHD8 蛋白的 表达，分析其与乙型肝炎 e 抗原（hepatitis B e antigen，HBeAg）、术后复发、转移等临床病理指标的相关性。 结果 免疫组织化学结果显示 CHD8 在乙肝相关性肝癌组织中的表达低于癌旁组织（P ＝ 0.00），临床病理特 征分析提示 CHD8 的表达与微卫星灶（P ＝ 0.00）、血管侵犯（P ＝ 0.00）、除肝及肺外其他部位转移（P ＝ 0.00） 明显相关，并与 HBeAg（P ＝ 0.04）、术后复发（P ＝ 0.01）、肺转移（P ＝ 0.04）具有相关性。结论 CHD8 基 因的表达与乙肝相关性 HCC 肝移植术后肿瘤转移复发相关，可能作为肝移植术后肿瘤复发转移的潜在标志物。